Naslov
Cascade reaction for the synthesis of D-fagomine precursor

Autori
Sudar, Martina ; Findrik Blažević, Zvjezdana ; Vasić-Rački, Đurđa ; Clapes, Pere

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th International Symposium of Environmental Management - Towards Circular Economy, Book of Abstracts / Katančić, Z., Koprivanac, N., Lončarić Božić, A., Kušić, H., Hrnjak-Murgić, Z. - Zagreb : Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, 2016, 63-63

ISBN
978-953-6470-75-4

Skup
4th International Symposium on Environmental Management, Towards Circular Economy, SEM 2016

Mjesto i datum
Zagreb, Hrvatska, 07.12.2016. - 09.12.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biocatalysis; cascade reaction; D-fagomine

Sažetak
Multistep enzyme-catalyzed reactions are gaining attention in the last decade, especially for the industrial production of both building blocks in pharmaceutical sector and bulk chemicals. These processes imply the use of different biocatalysts in tandem (Systems Biocatalysis) for the catalysis of multiple reactions without isolation of intermediates, mimicking biosynthetic pathways in living cells. Classical processes on the other hand, imply the isolation of intermediates in multi-step syntheses, which is both expensive and time consuming, they use too many chemicals, resulting in low yields and expensive end product. Since the need for the isolation of reaction intermediates is avoided in a cascade reaction, they result in higher yields and consume lower amount of chemicals. A mathematical model for the cascade synthesis of (3S, 4R)-6-[(benzyloxycarbonyl)amino]-5, 6- dideoxyhex-2-ulose was proposed in this work. The reaction consists of Cbz-N-3-aminopropanol oxidation catalyzed by horse liver alcohol dehydrogenase with coenzyme regeneration catalyzed with NADH oxidase and aldol addition of dihydroxyacetone to N-Cbz-3-aminopropanal catalyzed by D-fructose-6-phosphate aldolase from Escherichia coli (Fig 1). The mathematical model enables reaction prediction as well as detection of bottlenecks in optimization of the cascade system.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

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