Naslov
Standardization of molecular monitoring of bcr-abl fusion transcript according to International Scale

Autori
Horvat, Ivana ; Radić Antolic, Margareta ; Sertić, Dubravka ; Müller, Martin ; Zadro, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica / - Zagreb, 2009, 143-143

Skup
6. hrvatski kongres medicinskih biokemičara s međunarnodnim sudjelovanjem

Mjesto i datum
Supetar, Hrvatska, 30.09.2009. - 04.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bcr-abl fusion transcript; International Scale; standardization

Sažetak
Introduction: The most frequent method for monitoring therapy in chronic myeloid leukemia patients was qualitative PCR. Development of quantitative real-time PCR enables nowadays relative bcr-abl quantification. The result depends on the probe and housekeeping gene selection as well as in the standards used. For this reason the subproject “Molecular Monitoring“ within the European Leukemia Net intends to standardize the results obtained in different laboratories. Materials and methods:The aim of the study was to prepare 30 RNA samples that homogenously cover a range of > 3 logs between 0.001 and 10% bcr-abl, to perform the quantification by the method routinely used in laboratory and to send the same samples to the reference laboratory of the Mannheim University Hospital in order to obtain conversion factor for expression of results according to International Scale (IS). The procedure started with the reception of 4 lysates with different dilutions of bcr-abl positive WBC in healthy donors WBC from reference laboratory. Results: Quantification of bcr-abl was performed by the method routinely used in laboratory and the results sent to reference laboratory revealed the preliminary conversion factor of 0.7830. Next, 30 RNA samples were prepared in 6 aliquots, 2 x 107 WBC each. Bcr-abl quantification was performed in 3 aliquots. Other 3 aliquots together with the obtained results were sent to the reference laboratory where the same procedure was repeated. Comparison of results from both centers revealed the final conversion factor of 0.3911 that generates the results according to IS. Conclusion: Standardization enables the expression of results according to IS and comparability of results within institutions. Monitoring of dynamics of the change in bcr-abl fusion transcript quantity enables proper disease monitoring and decision in treatment options.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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