Naslov
Thrombosis in MPN patients and gene frequency of human platelet antigens

Autori
Horvat, Ivana ; Radić Antolić, Margareta ; Rončević, Pavle ; Serventi-Seiwerth, Ranka ; Zadro, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
MPN&MPNr-EuroNet Tenth Meeting "Biology of sporadic and Hereditary Myeloproliferative Diseases" : abstracts / - , 2015, 50-50

Skup
MPN&MPNr-EuroNet Tenth Meeting "Biology of sporadic and Hereditary Myeloproliferative Diseases"

Mjesto i datum
Zagreb, Hrvatska, 09.04.2015. - 10.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Thrombosis ; MPN ; human platelet antigen

Sažetak
Thrombosis is the most common complication in MPN patients. Besides hereditary thrombophilia factors, polymorphisms in human platelet antigens (HPA) may be associated with increased risk for thrombosis. The aim of this study was to investigate HPA genotype frequencies and association of genotype with risk for thrombosis in a group of JAK2 V617F positive MPN patients. The study included 99 JAK2 V617F positive MPN patients (39 PV, 41 ET, 19 MF and 3 MPN/MDS), 40 male and 59 female. Median age at diagnosis was 58 years (range 14-85 years). ASO PCR was performed for JAK2 V617F according to Baxter et al, Lancet 2005. Genotype analysis was done for hereditary thrombosis risk factors (FV Leiden and FII G20210A using PCR-RFLP, Zöller et al, J Clin Invest 1994) and for HPA -1, -2, -3, -5 polymorphisms (Real-time PCR, Ficko et al, Transfus Med 2004). The study included observation of additional risk factors including hypertension, diabetes, smoking, alcohol consumption and lipemia. Half of patients (50/99) had at least one additional risk factor. Screening for thrombophilia risk factors resulted in 3/99 heterozygotes for FV Leiden and 3/99 for FII G20210A. HPA -1, -2, -3 and -5 gene frequencies in these patients were similar to those in healthy Croatian population (Pavic et al, Transfus Med 2010). Patients were divided in 2 groups, group 1 (N=26) with and group 2 (N=73) without thrombotic event. There was no statistical difference in gender, age, diagnosis, hereditary and additional risk factors between those groups. HPA-5 genotype frequency was for group 1 a/a - 0.96, a/b - 0 and b/b - 0.04 and for group 2 it was a/a - 0.77, a/b - 0.23 and b/b - 0 (P=0.036). On the contrary, genotype frequency for HPA-1, HPA-2 and HPA-3 showed no statistical difference between two groups. In conclusion, HPA-5 genotype could have impact on risk for thrombotic event in MPN patients. Further investigations should be done in larger study groups.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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