Pregled bibliografske jedinice broj: 845405
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.) // Silva Fennica, 50 (2016), 5; 1-9 doi:10.14214/sf.1674 (podatak o recenziji nije dostupan, prethodno priopćenje, znanstveni)
CROSBI ID: 845405 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)
Autori
Ćelepirović, Nevenka ; Karija Vlahović, Monika ; Dounavi, Aikaterini ; Ivanković, Mladen
Izvornik
Silva Fennica (0037-5330) 50
(2016), 5;
1-9
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prethodno priopćenje, znanstveni
Ključne riječi
SSR markers ; nuclear microsatellites ; Juglans regia L. ; multiplex PCR
Sažetak
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube. In this study, we combined existing simple sequence repeat (SSR) markers (nuclear microsatellites) in the novel combination of multiplex PCR to study the population genetics of common walnut from Croatia. From twenty one tested SSR markers, eleven produced satisfactory results in one multiplex PCR. Population genetic results achieved from 15 samples of Croatian common walnut showed moderate genetic variability (average value: He 0.473 ; Ho 0.568). Our multiplex PCR allowed cost effective work concerning chemicals, plastic ware, device, and working time producing optimal results. The optimized multiplex PCR represented the best combination of eleven SSR primers for genotyping common walnut in a single PCR reaction.
Izvorni jezik
Engleski
Znanstvena područja
Šumarstvo
POVEZANOST RADA
Projekti:
024-0242108-2099 - Oplemenjivanje i šumsko sjemenarstvo (Ivanković, Mladen, MZOS ) ( CroRIS)
Ustanove:
Hrvatski šumarski institut, Jastrebarsko
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
