Naslov
Conformations of circular condensed DNA bundles in bacteriophages

Autori
Šiber, Antonio

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Christmas Biophysics Workshop Book of Abstracts / D’Adamo Giuseppe ; Micheletti, Cristian - Trst, 2015, 5-5

Skup
10th Christmas Biophysics Workshop

Mjesto i datum
San Daniele del Friuli, Italija, 14.12.2015. - 15.12.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
virus; DNA; elasticity

Sažetak
Some sort of DNA ”compression” is required to pack the long DNA strand in a small space, such as the cell nucleus or bacteria. Viruses have, in particular, developed several strategies to physically compress the DNA genome molecule. In presence of basic proteins [1] or multi-valent counterions, DNA can be ”condensed”, i.e. brought to a state where it self-attracts [2]. When condensed in confinement, e.g. in virus protein coatings (capsids), it is known that the, sufficiently short, DNA also assumes toroidal conformations, but the free energy balance is in that case additionally complicated by the adsorption energy (DNA capsid interaction) and by the presence of the capsid [3]. It has been proposed in the literature that the, sufficiently long DNA, may condense in conformations which are non- toroidal, i.e. which do not have the cylindrical axis of symmetry [4]. I will present a model calculation that accounts for non-toroidal conformations of DNA condensed in confinement. The model reproduces conformations that were previously predicted [4], but also several intriguing conformations that were never predicted in the context of viruses. References [1] A. J. Perez-Berna, S. Marion, F. J. Chichon, J. J. Fernandez, D. C. Winkler, J. L. Carrascosa, A. C. Steven, A. Siber, and C. San Martin, Nucl. Acids Res. 43, 4274 (2015). [2] J. Ubbink and T. Odijk, Europhys. Lett. 33, 353 (1996). [3] A. Leforestier, A. Siber, F. Livolant, and R. Podgornik, Biophys. J. 100, 2209 (2011). [4] N. V. Hud, Biophys. J. 69, 1355 (1995)

Izvorni jezik
Engleski

Znanstvena područja
Fizika

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