Naslov
Kinetics of selected cytokines and chemokines in patients with Puumala virus infection

Autori
Svoboda, Petra ; Cvetko Krajinović, Lidija ; Čikeš, Petra ; Topić, Antea ; Bosnar, Martina ; Eraković Haber, Vesna ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / - Zagreb, 2016, 50-50

Skup
2016 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Ogulin, Hrvatska, 14.10.2016. - 15.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Hemorrhagic fever with renal syndrome; Hantaviruses; Croatia; multiplexed immunoassay; innate immunity; acute disease

Sažetak
Objective: Hemorrhagic fever with renal syndrome (HFRS) is a viral disease caused by hantaviruses, however, it is primarily considered as an immune-mediated disease. Monocytes/macrophages (mo/ma) are important immune cells and are considered one of the target cells for hantaviruses. The aim of our study was to analyze the mo/ma related cytokines/chemokines profile in sera of HFRS patients infected with Puumala virus (PUUV) according to the severity of their clinical picture and in correlation with some laboratory parameters. Methods: The study included 34 HFRS patients with a serologically confirmed PUUV diagnosis, hospitalized in 2014 in Zagreb, Croatia. Patients' serum samples were taken in two acute time points – upon arrival at the hospital and before discharge. Luminex technology of (multiplexed) immunoassay with magnetic beads was used for 16 analytes, namely: IL-1β, IL-1RA, IL-12(p70), IL-15, IL-18, IL-23, IL-27, MIF, CCL2, CCL3, CCL4, CCL5, CCL7, CCL20, CXCL12a, TGF-β1. Results: The results showed, suppression of the early acute immune response to PUUV - some pro-inflammatory cytokines were down-regulated or absent. CCL4, IL-1RA, IL-18 and MIF were elevated in both phases compared to the controls, while CCL5 was downregulated in the first and upregulated in the second phase. In opposite, low levels of IL-15 were recorded in the first serum and not in the second serum sample. Correlations with some laboratory parameters revealed strong positive correlations of CCL5 and TGF-β1 with platelets in early acute mild form and positive CCL5 and negative CCL2 correlations with platelets for late acute moderate HFRS form. Conclusions: Some differences in the levels of monocytes/macrophages related cytokines/chemokines in HFRS patients were detected in patients with mild in comparation to the patients with moderate clinical picture and correlation with several clinical laboratory parameters were observed as well.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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