Naslov
Analysis of intestinal intraepithelial lymphocyte populations in experimental Trichinella spiralis infection of mice

Autori
Božić, Frane ; Marinculić, Albert ; Duraković, Emir

Izvornik
Folia Parasitologica (0015-5683) 47 (2000), 1; 55-59

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Analysis of intestinal intraepithelial lymphocyte populations in experimental Trichinella spiralis infection of mice

Sažetak
The potential role of intestinal intraepithelial lymphocytes (i-IELs) in the generation of host protective immunity after helminth infection was investigated using the Trichinella spiralis (Owen, 1835)/mouse model. In this study we found a significant rise of TCR gama delta i-IELs (P < 0.001) concurrent with the jejunal goblet cells (GC) hyperplasia in T. spiralis-infected C57BL mice on day 4 p.i. However, no direct relationship between the kinetics of the increase in TCR gama delta i-IELs and T. spiralis expulsion was observed in infected mice. Taken together, these results implicate that gama delta i-IELs probably perform a unique functions related to the regulation of the GC proliferation accompaining T. spiralis gut infection. As is known, these TCR gama delta i-IELs may release mediators or growth factors that in turn influence GC differentiation. With the use of dexamethason (DEX), a potent anti-inflammatory agent which also induces apoptotic cell death in i-IELs, we have confirmed that the expulsion of T. spiralis from the mouse gut is accompained by an inflammatory response. Indeed, the GC are clearly involved in these phenomena, apparently under the regulation by TCR gama delta i-IEL-mediated responses, since DEX abrogated GC proliferation in T. spiralis-infected C57BL mice and subsequently augmented adult worm burden. Our data also show that the rejection of adult worms starts concurrently with a significant increase in TCR alfa beta and CD8+ i-IELs (P < 0.05 and (P < 0.01, respectively), namely by day 7 p.i. At the same time, CD4+ cells significantly decreased (P < 0.05) in the intestinal epithelium of T. spiralis-infected, vs. uninfected mice. These results may indicate that the TCR alfa beta and CD8+ i-IELs act as effectors of anti-T. spiralis defence reactions. The implications of these findings for the potential role of intestinal intraepithelial CD8+ and TCR alfa beta cells in the pathogenesis of the intestinal lesions during T. spiralis gut infection are discussed.

Izvorni jezik
Engleski

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