Naslov
Escherichia coli in marine water: comparison of methods for the assessment of recreational bathing water samples

Autori
Vukić Lušić, Darija ; Jozić, Slaven ; Cenov, Arijana ; Glad, Marin ; Bulić, Marko ; Lušić, Dražen

Izvornik
Marine pollution bulletin (0025-326X) 113 (2016), 1-2; 438-443

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
coastal water quality ; Escherichia coli ; method comparison ; ISO method ; Chromocult® Coliform Agar ; alternative method

Sažetak
Bathing Water Directive (2006/7/EC) specifies two reference methods for Escherichia coli detection: ISO 9308-1 and 9308-3. The revised ISO 9308-1 is recommended only for waters with a low bacterial background flora. Considering the extended time needed for analysis and, generally, the lack of experience in using ISO 9308-3 in the Mediterranean, the suitability of ISO 9308-1 for the examination of E. coli in bathing water was evaluated. The present study was aimed at a comparison of data obtained by the reference method in seawater samples (110 beaches, N=477) with data received from six alternative methods. Results show that recently used TSA/TBA method may overestimate E. coli numbers in marine waters. The temperature modified ISO 9308-1 (44 °C) did not significantly alter the results, but outperformed the antibiotic supplemented agar at reducing non-E. coli bacteria on the plates, allowing the use of the respective method for monitoring coastal water.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita, Prehrambena tehnologija

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