Naslov
MULTI-RESPONSE OPTIMIZATION OF ULTRASOUND-ASSISTED GLYCEROL EXTRACTION OF BIOACTIVE BERBERINE FROM Berberis vulgaris

Autori
Dulić, Marina ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
BOOK OF ABSTRACTS • KNJIGA ABSTRAKTA The International Bioscience Conference and the 6th International PSU – UNS Bioscience Conference IBSC 2016 / Mimica-Dukić, Neda ; Pajević, Slobodanka ; Mandić, Anamarija - Novi Sad : University of Novi Sad, Faculty of Sciences, 2016, 217-217

ISBN
978-86-7031-364-4

Skup
The International Bioscience Conference and the 6th International PSU – UNS Bioscience Conference IBSC 2016

Mjesto i datum
Novi Sad, Srbija, 19.09.2016. - 21.09.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Berberis vulgaris ; berberine ; radical scavenging activity ; response surface methodology.

Sažetak
INTRODUCTION: Barberry (Berberis vulgaris L., Berberidaceae) is a well known medicinal plant with a long history of medicinal and nutritional use in Europe, Asia and America. It has various therapeutic effects, mostly related to its isoquinoline alkaloids, particularly berberine. OBJECTIVES: The aim of this study was to examine the efficiency of ultrasound-assisted extraction of berberine from Berberis vulgaris using glycerol as non-toxic, biodegradable solvent from renewable sources. METHOD / DESIGN: Experiment was planned using Box-Behnken design. The range of design parameters (independent variables) was: temperature (X1, 20-80C), glycerol concentration (m/m) (X2, 10-90%) and ultrasonication power (X3, 72-720 W). Berberine content of the extracts was determined using RP-HPLC-DAD, while radical scavenging activity of the extracts (RSA) was determined spectrophotometrically, using DPPH free radical (dependent variables). Response-surface methodology was used to find the relationship between dependent and independent variables. RESULTS: The relationship between variables can bestbe described by quadratic models. The selected quadratic models were highly significant (P > 0.01) and had r2 values over 0.93. The most important extraction variables were temperature and glycerol content. They significantly (P > 0.05) influenced berberine concentration and RSA both as linear and quadratic terms. Furthermore, ultrasonication power had a significant (P< 0.05) effect on berberine concentration as a quadratic term, as well as in interaction with the extraction temperature. The solutions to the quadratic equations have shown that the best extraction conditions are (X1, X2, X3) for berberine extractions are 80 C, 50 % glycerol and 144 W, while the extracts showing best RSA were obtained using 80 C, 40 % glycerol and 720 W. The experimental data has confirmed these findings. CONCLUSIONS: The results demonstrate that ultrasonication-assisted extraction using glycerol is a fast and efficient method for preparation of extracts with notable berberine concentration and radical scavenging activity.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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