Naslov
THE EFFECT OF PROTEOLYTIC PROCESSING ON THE LOCALIZATION AND PHYSIOLOGICAL ACTIVITY OF THE Saccharomyces cerevisiae CELL WALL PROTEIN Scw4p

Autori
Teparić, Renata ; Grbavac, Antonija ; Kunštek, Sandra ; Mrša, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of Congress of the Croatian Society of Biochemistry and Molecular Biology "The Interplay of Biomolecules", HDBMB2014 / Katalinić, Maja ; Kovarik, Zrinka ; - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2014, 134-134

ISBN
978-953-95551-5-1

Skup
"The Interplay of Biomolecules", HDBMB2014

Mjesto i datum
Zadar, Hrvatska, 24.09.2014. - 27.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Scw4p; Kex2p; yapsins

Sažetak
Yeast cell wall contains proteins that are noncovalently (Scw- proteins) or covalently (Ccw-proteins) bound to β-1, 3-glucan, either through GPI-anchors and β-1, 6-glucan, or by alkali labile ester linkages between γ-carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir-proteins). It was previously shown that one of the most abundant Scw protein, Scw4p is partly also covalently linked to the cell wall and that non- covalently bound Scw4p underwent the proteolytic processing, while the covalently bound Scw4p was not processed. Such finding indicates that the proteolytic processing might determine the localization of these two forms of the protein. Proteolytic enzymes which might have a role in processing of Scw4p are Kex2p and a family of aspartic proteases called yapsins. To get a better insight in the processing of Scw4p, different mutations were introduced to SCW4 in the region of predicted processing sites. On the other hand mutant strain was constructed missing all yapsin proteases and Kex2p (5yps∆kex2). Native and mutated forms of Scw4p were expressed in kex2 yeast strain, strain with all yapsin genes disrupted (5yps∆) and strain with all proteases disrupted (5yps∆kex2) and Scw4p processing was examined. Furthermore, phenotypes of strains overproducing native and genetically modified Scw4p forms were examined.

Izvorni jezik
Engleski

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