Naslov
Antiaflatoxigenic effect of fullerene C60 nanoparticles

Autori
Kovač, Tihomir ; Šarkanj, Bojan ; Klapec, Tomislav ; Borišev, Ivana ; Kovač, Marija ; Nevistić, Ante ; Strelec, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 5th Croatian Congress of Toxicology with International Participation (CROTOX 2016)u Archives of Industrial Hygiene and Toxicology, Vol. 67, Supplement 1 / Šarić, Marko ; Kopjar, Nevenka - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 51-51

Skup
5th Croatian Congress of Toxicology with International Participation CROTOX 2016

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
aflatoxin B1 ; Aspergillus flavus NRRL 3251 ; fullerene C60 ; nanoparticles ; oxidative stress

Sažetak
This study examined the antifungal and antiaflatoxigenic effect of environmentally plausible concentrations of fullerene C60 nanoparticles (nC60) on aflatoxicogenic fungi Aspergillus flavus NRRL 3251. Fungi were grown in YES medium for 168 h at 29 °C in the presence of 0, 10, 50, and 100 ng mL-1 of nC60, and dry mycelia weight determination was used for antifungal activity estimation, while aflatoxin content in yeast extract sucrose (YES) media was used to determine the antiaflatoxigenic effect. Aflatoxin B1 content in YES media was determined by a dilute and shoot LC/MS-MS method validated and fitted for this purpose. In addition, oxidative status markers (TBARS, Cu, Zn- SOD, Mn- SOD, CAT, GPX, GR and GSH/GSSG ratio) in A. flavus mycelia were determined by standard biochemical assays. Fullerene C60 nanoparticles neither exhibited the antifungal activity against A. flavus NRRL 3251 at any examined concentration, nor did they cause any significant change in oxidative status markers. However, exposure of the fungus to 10 and 100 ng mL-1 nC60 significantly reduced aflatoxin B1 production. The present data clearly indicate the antiaflatoxigenic effect of nC60, but only a detailed examination of the transcription factors which regulate cellular response to oxidative stress and activate secondary metabolism in aspergilli could shed light on the exact antiaflatoxigenic mechanism.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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