Naslov
Nanoparticle-mediated interplay of chitosan and melatonin for improved wound epithelialisation

Autori
Blažević, Filip ; Milekić, Tamara ; Duvnjak Romić, Marieta ; Juretić, Marina ; Pepić, Ivan ; Filipović-Grčić, Jelena ; Lovrić, Jasmina ; Hafner, Anita

Izvornik
Carbohydrate polymers (0144-8617) 146 (2016); 445-454

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
melatonin ; chitosan ; nanoparticles ; wound healing ; scratch test ; keratinocytes

Sažetak
Herein, we propose an innovative approach to improving wound healing. Our strategy is to delivermelatonin locally at the wound site by means of lecithin/chitosan nanoparticles. We used four typesof chitosan that differed in terms of molecular weight and/or deacetylation degree. Melatonin encap-sulation efficiency, nanoparticle size, zeta potential, biocompatibility and in vitro drug release werestudied as a function of the type of chitosan used in preparation. The nanoparticles were evaluatedin terms of their potential to promote wound epithelialisation via an in vitro scratch assay using ahuman keratinocyte (HaCaT) monolayer. The model wounds were treated with nanoparticle suspensionsat a chitosan concentration of 5 g ml−1, which was based on preceding cell biocompatibility studies.Nanoparticles prepared with different types of chitosan showed similar effect on the keratinocyte prolif-eration/migration. Nanoparticle-mediated interplay of chitosan and melatonin was shown to be crucialfor improved wound epithelialisation.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA