Naslov
Cyclodextrins as potent enhancers of olive secoiridoid extraction – the case of oleuropein in olive waste

Autori
Vitali Čepo, Dubravka ; Jug, Mario ; Albahari, Petra ; Živko, Tomislav, Koščica, Petra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of International Society of Antioxidants in Nutrition & Health Journal of ISANH Volume 3: Special Issue for Porto Polyphenols 2016 / - , 2016

Skup
10th World Congress on Polyphenols Applications: Porto Polyphenols 2016

Mjesto i datum
Porto, Portugal, 07.06.2016. - 09.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cyclodextrin ; olive pomace ; oleuropein ; inclusion complexes

Sažetak
Oleuropein (OLE) is generally the most prominent phenolic oleoside in Olea europaea and reaches maximal concentrations in young olives and olive leaves. Olive pomace contains significantly lower amounts of OLE but can also be used as widely accessible and valuable source of pharmacologically active polyphenols. Having in mind proven antioxidant, anti- inflammatory, anti-atherogenic, anti-cancer, antimicrobial, and antiviral effects of OLE, the main purpose of this investigation was to additionally optimize its extraction from olive pomace. For this purpose, different concentration levels of β-cyclodextrin (βCD), γ-cyclodextrin (γCD), hydroxypropyl-β cyclodextrin (HPβCD) and metyl-β-cyvlodextrin (MetβCD) were used during ultrasound assisted extraction of OLE from olive pomace. Obtained extracts were subsequently freeze-dried and analyzed for OLE content using RP HPLC-DAD. 12 times higher OLE yields were found in extracts containing 8g/L of βCD and ɣCD (532.9±49.2 mg/kg and 541.8±4.8 mg/kg respectively). Extraction with MetβCD also increased oleuropein yields (2.5 times) independently on concentration level of excipient in extraction solvent while HPβCD did not show positive effect on final OLE concentrations. Obtained results indicate that OLE forms inclusion complexes with βCD, ɣCD and probably MetβCD resulting in increased solubility of oleuropein in extraction solvent and improved resistance to degradation during extraction/freeze-drying.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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