Pregled bibliografske jedinice broj: 836327
Identification and antibiotic resistance of enterococci isolated from game meat sausages
Identification and antibiotic resistance of enterococci isolated from game meat sausages // Power of Microbes in Industry and Environment 2016
Krk, Hrvatska, 2016. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 836327 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Identification and antibiotic resistance of enterococci isolated from game meat sausages
Autori
Hulak, Nataša ; Žgomba Maksimović, Ana ; Tanuwidjaja , Irina ; Mrkonjić Fuka, Mirna
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
Power of Microbes in Industry and Environment 2016
Mjesto i datum
Krk, Hrvatska, 28.09.2016. - 01.10.2016
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
antibiotic resistance ; enterococcus ; game meat sausages
Sažetak
Fermented foods produced by traditional techniques represent an important vehicle for live bacteria to human gastrointestinal tract (GIT). These bacteria may carry genes coding for antibiotic resistance which may be transferred to the other commensal or pathogen bacteria in GIT. It has been reported that indigenous bacteria found in artisan products may possess intrinsic multi-resistance to antibiotics, a trait that has evolved in bacteria before antibiotics were even applied in medicine or husbandry. Furthermore, bacteria possess a remarkable ability to develop new resistances (acquired resistance) through many different mechanisms. As the data for antibiotic resistances of bacteria and interspecies diversity of enterococci from traditional fermented sausages is scarce, one of the main aims of our study was to identify enterococci of traditional sausages produced from deer (Cervus elaphus) and wild boar (Sus scrofa) meat and to analyse their susceptibility to different clinically important antibiotics. Many studies have based the identification of enterococci with 16S rRNA gen-specific primers. However, due to the fact that non-human sources may be atypical and usually do not meet the criteria used for standard characterization we have used the superoxide dismutase gene (sodA) for interspecies differentiation of enterococci. To test their antimicrobial susceptibility, agar disc diffusion method against 6 antibiotics was applied. By sodA PCR approach, we were able to confirm and to determine Enterococcus faecalis, E. faecium, E. durans, E. casseliflavus and E. gallinarum isolated on kanamycine esculine azid agar (KAA) from game meat sausages during the fermentation and ripening (0, 4, 7, 10, 20, 40 days). From total of 280 isolates, the resistance towards tetracycline 30 µg with 4 %, erythromycin 15 µg with 2.5 % and vancomycin 5 with 3 % was observed, as well as intermediate resistance for erythromycin 15 µg (57 %), chloramphenicol 30 µg (5 %) and tetracycline 30µg (2 %). In order to detect genes and where they are located (plasmid or chromosome), further study is necessary. Identification of enterococci on species level by multiplex PCR method with primers for unique sodA gene was a valuable tool for reliable identification of indigenous enterococci from game meat sausages.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Ustanove:
Agronomski fakultet, Zagreb