Naslov
ANTIFUNGAL AND ANTIAFLATOXIGENIC ACTIVITY OF COUMARIN DERIVATIVES
(ANTIFUNGALNI I ANTIAFLATOKSIKOGENI UČINAK DERIVATA KUMARINA)

Autori
Tomić, Marinko ; Buljubašić, Martin ; Kovač, Tihomir ; Šarkanj, Bojan ; Kovač, Marija ; Nevistić, Ante ; Pavić, Valentina ; Strelec, Ivica ; Molnar, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts International conference 16th Ružička days “TODAY SCIENCE – TOMMOROW INDUSTRY” / Jukić, Ante - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2016, 66-66

ISBN
978-953-7005-40-5

Skup
International conference 16th Ružička days “TODAY SCIENCE – TOMMOROW INDUSTRY”

Mjesto i datum
Vukovar, Hrvatska, 21.09.2016. - 23.09.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antiaflatoxigenic activity ; antifungal activity ; Aspergillus flavus NRRL 3251 ; coumarin derivatives
(antiaflatoksikogeno djelovanje ; antifungalno djelovanje ; Aspergillus flavus NRRL 3251 ; derivati kumarina)

Sažetak
Antifungal and antiaflatoxigenic effect of two series of coumarinyl thiosemicarbazides on Aspergillus flavus NRRL 3251 were studied. Fungi were grown in YES medium for 72 h at 29 °C in the presence of 0, 0.1, 1 and 10 µg mL-1 coumarinyl thiosemicarbazides, one series of compounds with substitution in position 7 of coumarin core and another with substitution in position 4 of coumarin core. Dry mycelia weight determination was used for antifungal activity estimation, while aflatoxin B1 content in YES media, determined by dilute and shoot LC-MS/MS technique, was used for antiaflatoxigenic effect estimation. Standard biochemical assays were used for oxidative status markers (TBARS, SOD, CAT and GPX) determination in A. flavus NRRL 3251 mycelia. Results showed that 7- substituted-coumarinyl thiosemicarbazides has better antifungal and antiaflatoxigenic activity than 4-substituted-ones. Among tested, the most prominent was compound 3, N-(4- chlorophenyl)-2-(2-((4-methyl-2-oxo-2H-chromen- 7-yl)oxy)acetyl)hydrazine-1-carbothioamide, which completely inhibited aflatoxin production at concentration of 10 µg mL-1. Oxidative stress response of A. flavus exposed to selected compounds, indicate modulation of oxidative stress as possible reason of aflatoxin production inhibition.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

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