Naslov
Genetic instabilities of the E-cadherin and APC genes in renal cell carcinoma

Autori
Pećina-Šlaus, Nives ; Gall-Trošelj, Koraljka ; Kapitanović, Sanja ; Radić, K. ; Pavelić, Krešimir ; Pavelić, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of 3rd International Conference on Signal Transduction / Đikić, I. ; Husnjak, K. - Zagreb, 2002

Skup
3rd International Conference on Signal Transduction

Mjesto i datum
Cavtat, Hrvatska, 17.05.2002. - 23.05.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
genetic instability; APC gene; renal carcinoma

Sažetak
The roles of adenomatous polyposis coli, APC and E-cadherin, CDH1 genes were investigated in renal cell carcinoma (RCC). Forty-five human renal cell carcinomas were tested for APC gene instability by polymerase chain reaction/loss of heterozygosity (LOH) using RFLP method. E-cadherin gene was analyzed by PCR amplification of tetranucleotide marker (D16 S752) and the alleles were visualized by PAGE/silver staining or Spreadex gels (Elchrom Scientific). The overall proportion of LOH cases of the APC gene was 37.5% (9/24) based on two polymorphic markers (Rsa I in exon 11 and Msp I in exon 15). D16 S752 marker linked to E-cadherin gene (informativity 91%) revealed 3 (7.5%) LOH cases. Interestingly, in 10% of RCC samples another type of genomic instability was detected, replication error (RER). Multivariate statistical analysis of samples informative for both APC and E-cadherin genes showed, that in this data set, LOH of the APC gene is correlated with advanced age and more severe TNM stages. Genomic instabilities of the E-cadherin gene, on the other hand, appear to be correlated with younger age groups and less severe TNM stages. Our results suggest that alterations, both in APC and E-cadherin genes, are involved in evolution and progression of RCC. Microsatellite genetic instability of the E-cadherin gene indicates that another cellular mechanism, mismatch repair, is targeted in RCC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA