Naslov
Anisakis pegreffii (Nematoda, Anisakidae) total antigen exerts oxidative stress and modulate the expression of biomolecular markers related to inflammation and cancer in human cell line

Autori
Messina, Concetta M ; Santulli, Andrea ; Boban, Mate ; Orhanović, Stjepan ; Bušelić, Ivana ; Mladineo, Ivona

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 17th International Conference on Diseases of Fish and Shellfish / Mladineo, Ivona - Las Palmas, 2015, 422-422

Skup
17th EAFP International Conference on Diseases of Fish and Shellfish

Mjesto i datum
Las Palmas de Gran Canaria, Španjolska, 07.09.2015. - 11.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
human normal fibroblast cell lines; reactive oxygen species; gene expression

Sažetak
Humans can become accidental host after consumption of raw or thermally inadequately treated fishery products that are contaminated by alive third-stage (L3) Anisakis larvae. Different types of anisakiasis have been recognised ; gastric, intestinal, ectopic or gastroallergic anisakiasis, in hypersensitized patients. In countries where both the prevalence of human cases and general awareness of this zoonosis is high, there have been described rare cases of bowel carcinomas associated to the isolated larvae. It is still unclear if two aetiologies, e.g. anisakiasis and carcinoma are related or accidental incidences, but it is indicative that in all reported cases, neoplasia and embedded larvae shared the common site affected by chronic inflammation. In line with this, the aim of our study was to test the effect of A. pegreffii total antigen (ATA) on human normal fibroblast cell lines HS-68 and subsequently measure the level of oxidative stress (ROS), inflammatory and carcinogenic markers (Hsp70, p53, c-fos, c- jun and TNF). Two types of ATA were used ; the first obtained from parasite excretory/ secretory production (ES), and the second obtained by whole-nematode ethanol extraction (CE). Preliminary results evidenced that cells have undergone a progressive reduction in vitality after ATA stimulation in dose and time dependent matter, but the effect differed in respect to the antigen product applied. Similarly, ROS was significantly increased compared to control cell line, and highly correlated to cell vitality. Expression of p53, c-foc and c-jun, quantified by q-PCR was congruent to immunoblotting detection of Hsp70 and TNF at protein level, all being significantly upregulated in affected cells. This suggests that Anisakis total antigen products can indeed trigger two pathways ; the first one is oxidative stress pathway characterized by inflammatory and apoptotic pattern in cells subjected to Anisakis excretory/ secretory products, while the other is more toxic effect characterized by induction of carcinogenic markers and blockage of inflammation and apoptosis in cells subjected to parasite crude extract.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Biotehnologija

POVEZANOST RADA