Naslov
Development of a LC/MS/MS method for the comprehensive assessment of of macrolide antibiotics in the aquatic environment

Autori
Senta, Ivan ; Krizman, Ivona ; Terzić, Senka. ; Ahel, Marijan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SETAC Europe 26th Annual Meeting Abstract Book / - Nantes : Society of Environmental Toxicology and Chemistry (SETAC), Europe Office, 2016, 139-140

Skup
26th Annual Meeting of the Society of Environmental Toxicology and Chemistry (SETAC)

Mjesto i datum
Nantes, Francuska, 22.05.2016. - 26.05.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
macrolide antibiotics; LC-MS-MS; solid-phase extraction (SPE); environmental samples

Sažetak
Macrolide antibiotics are among the most popular types of antimicrobials. As a concequence, a number of studies in the literature addressed the issue of environmental exposure to these emerging contaminants in municipal wastewaters and ambient waters. Macrolide antibiotics are released from the human body after therapeutic application mainly unchanged, however, several literature reports indicated that macrolides can be transformed in the environment either via SETAC Europe 26th Annual Meeting Abstract Book 139 138 SETAC Europe 26th Annual Meeting Abstract Book microbial or photochemical degradation, yielding a number of transformation products. Nevertheless, most of the existing exposure data refer only to the parent compounds, while the information on possible macrolide intermediates and/or transformation products in the aquatic environment are very scarce. One of reasons is the lack of analytical methods for the comprehensive assesment of macrolide-derived compounds in aqueous samples. Therefore, the aim of this work is to develop a multiresidue method for the simultaneous determination of several prominent macrolide antibiotics (azithromycin, clarithromycin, erythromycin and roxithromycin) along with their intermediates and transformation products using liquid chromatography tandem mass spectrometry (LC/MS/MS). The method development comprised improvement of the chromatographic separation of macrolide compounds using a new type of reversed-phase stationary phase, which combines octadecyl and phenyl moieties, and optimization of enrichment of target compounds from aqueous phase using Oasis HLB and mixed-bed stationary phases. The developed method was employed for the analysis of macrolide-derived residues in real wastewater and ambient water samples, collected in the area of the city o Zagreb. It was shown that intermediates and/or transformation products significantly contribute to the overall mass balance of macrolide-derived compounds. This indicates that the risk associated with the use of macrolide compounds in the aquatic environment should include assessment of possible effects caused by their transformation products and intermediates from their production.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA