Naslov
Impact of differently coated silver nanoparticles on antioxidative stress response in Nicotiana tabacum seedlings

Autori
Majsec, Kristina ; Tudić, Antonio ; Cvjetko, Petra ; Balen, Biljana ; Peharec Štefanić, Petra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Plant Biology Europe EPSO/FESPB 2016 congress / - , 2016

Skup
Plant Biology Europe EPSO/FESPB 2016 congress

Mjesto i datum
Prag, Češka Republika, 26.06.2016. - 30.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
silver nanoparticles; oxidative stress; tobacco seedlings

Sažetak
Nanotechnology has led to series of innovations in medicine, cosmetics, food industry and many other fields. Due to their size, nanoparticles (NPs) have distinct properties which make them useful in wide range of applications. This increased production and consumption of NPs has led to an increased NP release to the environment, without clear assessment of ecological and public health consequences. Silver nanoparticles (AgNPs) are now commonly used in medical applications, paint and textile manufacturing, due to their antimicrobial and antifungal properties. AgNP toxicity has been established in many species ranging from bacteria, plants, aquatic and terrestrial animals. However, it is still unclear whether their toxicity is a consequence of Ag ions release or the very nanoparticle form. Furthermore, AgNP toxicity may depend on coating substances used in NP production. In this work we have studied the impact of citrate (negatively charged), polyethylene glycol (PEG, neutral) and branched polyethylenimine (BPEI, positively charged)-coated AgNPs on tobacco seedlings in comparison with ionic Ag (AgNO3). Tobacco seedlings were grown from seeds in liquid MS and ½ MS medium supplemented with citrate-AgNP, PEG-AgNP, BPEI-AgNP and AgNO3 in wide concentration range (0, 25, 50, 75, 100, 125, 150, 180 µM). After 10 days seedlings were harvested for analysis. Activities of antioxidative enzymes APX, CAT, PPX and SOD were measured together with malon-dialdehyde and carbonyl content. Furthermore, we used fluorescence spectroscopy to detect superoxide in intact cells using dihydroethydium as fluorescent probe. Comet assay showed us DNA damage and total silver content of whole seedlings was analysed by inductively coupled plasma-mass spectrometry (ICP-MS). All three coated AgNPs show similar toxicity symptoms on macroscopic level: stunted growth and especially root growth inhibition. However, antioxidative stress response and principal component analysis reveal some interesting differences.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA