Naslov
A study of genetic variability of human parainfluenza virus type 1 in Croatia, 2011-2014

Autori
Košutić Gulija, Tanja ; Slović, Anamarija ; Ljubin-Sternak, Sunčanica ; Mlinarić-Galinović, Gordana ; Forčić, Dubravko

Izvornik
Journal of Medical Microbiology (0022-2615) 65 (2016), 8; 793-830

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HPIV1 ; molecular epidemiology ; haemagglutinin-neuraminidase ; fusion protein ; phosphoprotein

Sažetak
Molecular epidemiology of human parainfluenza viruses type 1 (HPIV1) was investigated. Samples were collected from patients hospitalized in Croatia during the three consecutive epidemic seasons (2011- 2014). Results indicated co-circulation of two major genetic clusters of HPIV1. Samples from current study refer to clades II and III in a phylogenetic tree of hemagglutinin- neuraminidase (HN) gene. Additional phylogenetic trees of fusion (F) and phosphoprotein (P) gene confirmed the observed samples topology. Analysis of nucleotide diversity of entire P, F and HN genes demonstrated similar value ; 0.0255, 0.0236, and 0.0237, respectively. However, amino acid diversity showed F protein to be the most conserved, while P protein was the most tolerant to mutations. Potential N- and O- glycosylation sites suggested that HPIV1 HN protein is abundantly glycosylated, and a specific N-glycosylation pattern could distinguish between clades II and III. Analysis of potential O-glycosylation sites in F protein indicated that samples from this study have a two O-potential glycosylation sites while publicly available sequences have five potential sites. This study provides data on molecular characterization and epidemic pattern of HPIV1 in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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