Naslov
Detekcija bakterijskih infekcija metodom lančane reakcije polimerazom (PCR) u korionskim resicama spontano pobačenih ljudskih plodova
(Detection of bacterial infection by polymerase chain reaction (PCR) in chorionic villi from human spontaneous miscarriage)

Autori
Matovina, Mihaela ; Husnjak, Koraljka ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
PROGRAM I KNJIGA SAŽETAKA 6. HRVATSKI KONGRES KLINIČKE MIKROBIOLOGIJE S MEĐUNARODNIM / - Zagreb : INOVITA, 2002, 162-163

Skup
6. HRVATSKI KONGRES KLINIČKE MIKROBIOLOGIJE S MEĐUNARODNIM SUDJELOVANJEM

Mjesto i datum
Zagreb, Hrvatska, 15.05.2002. - 17.05.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
-

Sažetak
Incidence of spontaneous miscarrieges in clinicaly recognised pregnancies is about 12-15%. Genetic abnomalities have been determined as the leading cause of spontaneous miscarriages. In the first trimester of pregnancy, which comprises the period between conception and 13th gestational week, chromosomal aberations were the cause of 30-60% of miscarriages. Intrauterine infections, among others, are considered as the possible cause of miscarriages. We explored the presence of bacterias in general and bacterias Ureaplasma urealyticum and Mycoplasma hominis specifically in the placental tissue in order to determine whether they are involved in the etiology of spontaneous miscarriage. Our sample consisted of 82 women who had one or more spontaneous miscarriages and the material consisted of placental chorionic villi gathered after the evacuation of miscarriages from uterus. Mean age of our female patients was 32 (17-46) years and mean gestational age was 10 (4-19) weeks. The material was karyotyped and DNA isolated from the tissue was anaiyzed by polymerase chain reaction (PCR). All samples were tested with consensus primers for 16S rRNA, which recognize wide spectrum of bacteria to detect possible bacterial infection. The samples were also tested with U. urealyticum and M. hominis specific primers. Genetic abnormalities were detected in 28 of 72 cases (39%) and in 44 cases the karyotype was normal which is in accordance with other literature findungs. We were unable to determine karyotype in 10 cases. By means of PCR with the primers for 16S rRNA we detected bacterial infection in 5 samples (6%) and with specific primers U. urealyticum and M. hominis were not detected in any of samples. These results are suggesting that U. urealyticum and M. hominis are not involved in the etilogy of spontaneous miscarriage but positive results obtained with primers for 16S rRNA suggest that we have to consider bacterial infections as the possible cause of miscarriages.

Izvorni jezik
Engleski

Napomena
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