Naslov
Development and characteristics of working HBsAg standard: a potential national standard

Autori
Lovrić, Manuela: Bosanac, Ivanka ; Jukić, Irena ; Mihaljević, Ivanka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Vox Sanguinis / - , 2005, 99-99

Skup
XVth European regional Conference Europe

Mjesto i datum
Atena, Grčka, 02.07.2005. - 06.07.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Croatian HBsAg standard

Sažetak
High sensitivity assays are used on donor blood testing for HBsAg. The quality of blood testing at the national level can be upgraded by using a national HBsAg standard to achieve uniform test sensitivity. Aim of study: To develop a HBsAg standard at a concentration of 0.2 IU/mL, on the model of the British HBsAg standard, stable in liquid state for one year at +4 ºC. The standard should be used as a working standard in blood testing for HBsAg in the Croatian transfusion service. Material and methods: The plasma collected from five HBsAg positive donors was converted to serum by use of thrombin. The sera obtained were filtered in sterile conditions and diluted with distilled water at a 1:4 ratio. The sera were individually and then pooled aa adjusted to a concentration of 100 IU HBsAg by use of PBS-BSA, 5% (preservative 0.05%, Bronidox), according to the method of Ferguson et al. from 2001. A modification of Murex-Abbott HBsAg test, version 3 GE 34/36, was used on plotting calibration curve and determination of HBsAg concentration against WHO International Standard of 100 IU/mL. Results: The stability of HBsAg standard 0.2 IU/mL was assessed for 12 months by use of Murex HBsAg test on automated and semiautomated patient blood testing, yielding an S/CO value of 5.43±0.85. Also, the HBsAg standard 0.2 IU/mL was assessed by the same test on an Ortho Summit Processor analyzer for 6 months, yielding an S/CO value of 4.95±0.67. Single testing of the HBsAg standard 0.2 IU/mL was performed at 27 transfusion medicine laboratories, in 16 of them by use of Axsym Abbott test, in 8 by IMx Abbott test, in 2 by Ortho HBsAg Elisa 3 test, in 1 by Hepanostika Biomerieux test, and in 1 by Abbott Prism HBsAg test. The mean S/CO value was 2.78, 5.3, 8.0, 3.0 and 9, 5 for Axsym, Imx, Ortho, Hepanostika and Prism test, respectively. Conclusion: The HBsAg standard 0.2 IU/mL, developed according to Ferguson et al., is stable in liquid state for at least 12 months at +4 ºC. The HBsAg standard 0.2 IU/mL showed an efficient S/CO ratio of 2-10 with the use of 10 different HBsAg tests, thus meeting the conditions required for a national HBsAg standard.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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