Evaluation of the cytotoxic activity of Hypericum spp. on human glioblastoma A1235 and breast cancer MDA MB-231 cells

Madunić, Josip; Matulić, Maja; Friščić, Maja; Hazler Pilepić, Kroata

doi:10.1080/10934529.2016.1206385

Pregled bibliografske jedinice broj: 823689

Evaluation of the cytotoxic activity of Hypericum spp. on human glioblastoma A1235 and breast cancer MDA MB-231 cells

Madunić, Josip; Matulić, Maja; Friščić, Maja; Hazler Pilepić, Kroata

Evaluation of the cytotoxic activity of Hypericum spp. on human glioblastoma A1235 and breast cancer MDA MB-231 cells // Journal of Environmental Science and Health, Part A, 51 (2016), 13; 1157-1163 doi:10.1080/10934529.2016.1206385 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 823689 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation of the cytotoxic activity of Hypericum spp. on human glioblastoma A1235 and breast cancer MDA MB-231 cells

Autori
Madunić, Josip ; Matulić, Maja ; Friščić, Maja ; Hazler Pilepić, Kroata

Izvornik
Journal of Environmental Science and Health, Part A (1093-4529) 51 (2016), 13; 1157-1163

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hypericum ; A1235 glioblastoma cells ; MDA MB-231 breast cancer cells ; cell viability inhibition

Sažetak
Cytotoxic activity of 16 Hypericum ethanolic extracts was evaluated by MTT assay on two human cancer cell lines, glioblastoma A1235 and breast cancer MDA MB-231. Morphology and the type of induced cell death were determined using light and fluorescence microscopy. The majority of Hypericum extracts had no significant cytotoxic effect on MDA MB-231 cells. Eight extracts exhibited mild cytotoxic effect on A1235 cells after 24 h incubation, ranging from 8.0% (H. patulum) to 21.7% (H. oblongifolium). After 72 h of treatment, the strongest inhibition of A1235 viability was observed for extracts of H. androsaemum (26.4-43.9%), H. balearicum (25.8-36.3%), H. delphicum (14.8-27.4%) and H. densiflorum (11.2-24.1%). Microscopic examination of cells showed apoptosis as the dominant type of cell death. Due to observed high viability of treated cells, we propose that cytotoxic effects of Hypericum extracts could be related to alternations/interruptions in the cell cycle.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

POVEZANOST RADA

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Josip Madunić (autor)