Naslov
Respiratory burst and phagocytic activity of peripheral blood leukocytes of the European sea bass (Dicentrarchus labrax) stimulated by three autochthonous and a commercially available bacteria

Autori
Radonić, Ivana ; Vrbatović, Anamarija ; Hrabar, Jerko ; Bušelić, Ivana ; Jozić, Slaven ; Mladineo Ivona

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 6th Croatian Congress of Microbiology / Roberto Antolović - Zagreb, 2016, 44-44

Skup
6th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15.06.2016. - 18.06.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
autochthonous bacteria; peripheral blood leukocytes; probiotic; sea bass

Sažetak
Use of probiotics in aquaculture has been studied during the last years, highlighting their role in stimulating immune response in the common and grass carp, orange-spotted grouper, hybrid tilapia, eel, cobia, rainbow trout and olive flounder, while less attention has been given to the Mediterranean aquaculture species as the European sea bass (Dicentrarchus labrax). Respiratory or oxidative burst (RB) is the rapid release of reactive oxygen species (superoxide radical and hydrogen peroxide) from different types of immune cells (neutrophils and monocytes), after their contact with pathogens. It is synchronised with the phagocytic activity (PA), as it occurs in phagocytes when they start degradation of internalised particles and bacteria. In order to assess cell-mediated immune response to potential probiotics, we have isolated three autochthonous bacteria from intestine of farmed sea bass ; Enterovibrio sp., Alteromonas sp. and Pseudoalteromonas sp., and compared their effect on peripheral blood leukocytes(PBL)in respect to a commercially available Lactobacillus casei. PBL were prepared using 51% iso-osmotic Percoll solution, seeded in 6-well plates with L- 15 medium (with 15% FCS and 1% penicillin/streptomycin. RB of cells was quantified by measuring the reduction of nitroblue trazolium(NBT ; 0.3% NBT was added to settled PBL, and cells were afterwards stimulated with four bacterial isolates by adding 108 CFU/ml for 1, 3, 5 and 12h. For dissolving of reduced NBT, 2N KOH and DMSO were added, stirred and OD were read by a spectrophotometer at 630 nm. PA was evaluated after stimulation with four bacterial isolates (108 CFU/ ml in culture media for 1, 3, 5 and 12 h. After washing of remaining bacteria, Saccharomyces cervasie type II suspension was added to PBL and left for 2h. Phagocytosis was interrupted by fixing of cell monolayer in methyl- alcohol, PBL were visualised by staining with Giemsa and numbered up to 100 per well to calculate the percentage of active cells. Cell- mediated immunity through RB and PA was evident after stimulation with all bacterial isolates, showing similar time-dependent kinetics. The best effect was induced by L. casei, although immunostimulation of autochthonous isolates showed to be also significant.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina, Biotehnologija

POVEZANOST RADA