Naslov
Airborne, dustborne and foodborne fungi in flooded and unflooded area in Croatia

Autori
Jakšić Despot, Daniela ; Kifer, Domagoj ; Šegvić Klarić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress of Microbiology with International Participation, Book of abstracts / Roberto Antolović and Domagoj Kifer (Technical editor) - Zagreb : Hrvatsko mikrobiološko društvo, 2016, 92-92

ISBN
978-953-7778-13-2

Skup
6th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15.06.2016. - 18.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
airborne fungi ; dustborne fungi ; foodborne fungi ; flooded area ; Aspergilli ; section Aspergillus ; section Circumdati ; section Versicolores ; section Flavi ; section Nigri

Sažetak
In order to investigate post-flood mycological patterns in Gunja compared to an unaffected area (Gornji Stupnik) 20 indoor airsamples (IAS), 5 outdoor airsamples (ODAS) and 10 dust samples (DS) were taken at 5 repaired (RH) and 5 unrepaired houses (UNRH) in Gunja, 5 control houses in Gornji Stupnik (SH) and 10 IAS and 2 DS in the elementary schools in Gunja (ESG) and Gornji Stupnik (ESS). Additionally, food samples (FS) were collected in both Gunja (FSG, N=10) and Stupnik (FSS, N=5). We used Dichloran 18%- Glycerol Agar (DG-18) and Malt Extract Agar (MEA) for AS, while for DS and FS Dichlorane Rose- Bengal Chloramphenicol Agar (DRBC) and DG- 18 were used. Mean concentration of aeromycota (cfu/m3) in Gunja in RH (2.1x103) was similar to ODAS (1.8x103 cfu/m3), while in UNRH concentration was significantly higher in IAS (4.6x103) but not in ODAS (3.6x103). Compared to both UNRH and RH, concentration of aeromycota in control area (SH) was significantly lower in IAS (460 cfu/m3) and ODAS (70). Similary, concentration of airborne fungi was significantly lower in ESS (212) than in ESG (1.6x103). Penicillium, Cladosporium and Aspergillus (sections Aspergillus, Circumdati, Nigri) genera were prevailing except in EES where Rhizopus sp. were frequently isolated (60%). Aspergilli (section Aspergillus) were present in all IAS (13-47%), section Nigri in RH and SH (13%), Circumdati in ESG (18%) and Versicolores in RH and SH (7 and 33%). High concentration of Aspergilli from the section Versicolores (5.7x103) indicated heavy contamination in one of the RH in Gunja. Mean concentration of fungi in DS (cfu/g) from RH (2.7x106) was higher than in UNRH (1.x105) and SH (1.3x105). Also, higher concentrations were observed in DS from ESG (1.2x105) than ESS (4.8x104). Composition of aeromycota corresponds to dustmycota, but Aspergilli (Versicolores) were more frequent in DS (40- 100%). In food samples mean concentration of fungi (cfu/g ) was higher in FSS (2x106) than in FSG (5x105) and dominated by Fusarium spp. in both FSG (75%) and FSS (80%). These results indicate that two years after major flood in Gunja concentration of airborne fungi is much higher comparing to unflooded area and significat source of aeromycota are unrepaired houses, heavily contaminated with secondary and tertiary colonizers. High concentration of airborne and dustborne fungi represent significant treath to humans due to inhalatory exposure to potential mycotoxin producers.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

Napomena
Acknowledgements: This work has been fully supported by Croatian Scientific Foundation under the project MycotoxA (IP-09-2014-5982)

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