Naslov
Species diversity and toxigenicity of airborne black Aspergilli

Autori
Jakšić Despot, Daniela ; Kocsubé, Sandor ; Bencsik, Ottó ; Kecskeméti, Anita ; Szekeres, András ; Vágvölgyi, Csaba ; Varga, Janos ; Šegvić Klarić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress of Microbiology with International Participation, Book of abstracts / Roberto Antolović and Domagoj Kifer (Technical editor) - Zagreb : Hrvatsko mikrobiološko društvo, 2016, 50-50

ISBN
978-953-7778-13-2

Skup
6th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 15.06.2016. - 18.06.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Black Aspergilli; fumonisin B2; fum1 gene; fum8 gene; cytotoxicity; genotoxicity; comet assay; A549

Sažetak
Black Aspergilli (section Nigri) are ubiquitous moulds which contaminate foodstuffs and occur in indoor and outdoor environments. These species may represent a health hazard due to their irritant, allergic and toxic effects. Recent studies show that black Aspergilli can produce several fumonisin isomers including FB2 which toxic effects are poorly explored. The purpose of this study was: 1) to determine airborne black Aspergilli based on calmodulin gene sequence (CaM) ; 2) to explore FB2 production capacity in culture microextracts by LC/MS accompanied with the presence of fum1 and fum8 genes involved in fumonisins biosynthesis ; 3) to assess the cytotoxic and genotoxic potential of FB2 compared to FB1 as well as to FB2-positive and -negative Aspergilli microextracts in human lung adenocarcinoma cells (A549). In A. welwitschiae (7/15) and A. niger (1/1) isolates presence of both fum1 and fum8 genes correlated with FB2 presence in culture microextracts, in concentrations from 127.79 ng/mL to 13467.74 ng/mL (A. welwitschiae) and 15.16 ng/ml (A. niger). Upon 24 h treatment with 100 µM both FB1 and FB2 slightly altered A549 cells viability (83.40% and 71.25%, respectively). Microextracts (mg of microextract per mL of culture media) prepared from A. tubingensis and A. piperis were the most cytotoxic (IC20= 0.400 ± 0.002 mg/mL and 0.62 ± 0.06 mg/mL, respectively) followed by A. welwitschiae (IC50= 0.246 ± 0.007 mg/mL). A. luchensis and A. niger extracts (0.8 mg/mL) decreased cell viability to 58.88 % and 88.80 %, respectively. Tail intensity (% of DNA in tail) was the comet assay parameter selected to evaluate DNA damage compared to control treatment (0.1% DMSO). After 24 h treatment both FB1 and FB2 (10 µM) caused significant DNA damage (0.35 % and 0.33 %, respectively) as compared to control (P<0.0001). Surprisingly, FB2 but not FB1 applied in 100 µM caused significant DNA. Aspergilli culture microextracts were applied in concentration (0.1 mg/mL) which did not significantly alter cell viability. FB2-negative extracts of A. tubingensis and A. piperis caused significant DNA damage (0.3 % and 0.14% respectively) as compared to control (P<0.0001) but not A. luchuensis (P=0.1273). FB2-positive extracts of A. welwitschiae and A. niger exerted lower genotoxic potential with tail intensity 0.08% and 0.04 %, respectively (P<0.0001). These results suggest that the other metabolites but not FB2 were responsible for cytotoxicity and genotoxicity of tested black Aspergilli.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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