Naslov
Changes in BV-2 microglia susceptibility to murine cytomegalovirus as a result of varying cultivation conditions

Autori
Jurdana, Kristina ; Marcelić, Marina ; Rački, Valentino ; Kučić, Natalia

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology

Mjesto i datum
Split, Hrvatska, 01.06.2016. - 04.06.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
microglia ; cytomegalovirus

Sažetak
Murine microglial BV-2 cell line is commonly used as a substitute for primary microglia. They are commonly cultivated in the Dulbecco’s modified eagle medium (DMEM) supplemented with fetal calf serum (FCS) and L-glutamine (L-G). This cellular environment is unable to imitate the one present in the central nervous system, thus causing morphological and functional changes to the microglial cells. Microglial cells are reactive by nature, and as such are affected by all changes in their environment. BV-2 microglia cells exhibited low susceptibility to murine cytomegalovirus (MCMV) in the first experiments we performed with the goal of establishing an in-vitro infection model. As a result, our aim was to determine whether the changes in the condition of cultivation can affect the susceptibility of BV-2 cells to MCMV infection. For the purposes of these experiments cells were grown in the FCS and L-G depleted cultivation medium. Researchers have demonstrated that FCS can alter the metabolic and immune state of cells due to its immunogenic nature. Furthermore, to achieve more natural microenviromental conditions ATP and tocopherol-α in various concentrations were added as a substitute. Results from our study show that manipulation of cultivation conditions, specifically FCS depletion, leads to morphological changes of BV-2 microglia cells. Complete FCS depletion resulted in increased susceptibility of this cell line for MCMV-infection. Varying FCS depletion also increased pro-inflammatory markers CD86 and iNOS, depending on the concentration. This indicates that the environmental changes that modulate the metabolic and immunological profile can cause an altered response of microglial cells to pathogens.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA