Naslov
Biomarker response analysis in fish within the SOLUTIONS project - Case study Holtemme

Autori
Deutschmann, Björn ; Seiler, Thomas-Benjamin ; Yang, Fangxing ; Markert, Nele ; Velki, Mirna ; Brack, Werner ; Hollert, Henner

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SETAC Europe 26th Annual Meeting: Abstracts / - , 2016

Skup
SETAC Europe 26th Annual Meeting

Mjesto i datum
Nantes, Francuska, 22.05.2016. - 26.05.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
SOLUTIONS; biomarker responses; brown trout

Sažetak
Close collaboration with several case studies is one of the main advantages in terms of the realization of biomarker response analysis within the 7th EU RTD Framework Programme project SOLUTIONS (Solutions for present and emerging pollutants in land and water resource management). By using the infrastructure of these studies, biomarker monitoring approaches can be performed easily. Under the lead of the Helmholtz-Centre for Environmental Research the small river Holtemme was investigated in an interdisciplinary approach. The stream has its source in the Mountain Harz region (Germany) and flows through a number of small and medium- sized cities in Saxony-Anhalt. Due to its low water-quantity and several anthropogenic point sources the Holtemme is a very valuable model site to assess the impact of pollution induced biomarker responses in aquatic organisms. 10 individuals of brown trout (Salmo trutta) were collected at each of 4 different locations including a “natural” reference site. Locations were downstream waste water treatment plants (WWTPs) and one location in a more agriculturally influenced river section. The utilized biomarker battery covered a broad range of proven endpoints which have been employed in several previous studies: genotoxicity in peripheral erythrocytes (micronucleus test), biotransformation enzyme activity (EROD, GST, CES), oxidative stress (TBARS, CAT) and biomarker of exposure to organophosphate and carbamate pesticides (AChE). To reduce time and sample consumption all common cuvette analysis for these endpoints were adapted to 96-well plate assays. For most of the investigated biomarkers significantly elevated/lowered values for the urban and agriculture-affected sampling sites could be demonstrated in comparison to the reference site. However, the results indicated no similar trend for all biomarker responses along the longitudinal profile of the River Holtemme, but the individual biomarkers gave “hot-spots” of response. Correlation analysis with chemical data might help to identify presumed stressors related to the different observed effects.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA