Naslov
DNA replication differences between diffuse large B-cell lymphoma subtypes

Autori
Sasi, Biljana

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
03.07

Godina
2015

Stranica
29

Mentor
Korać, Petra

Ključne riječi
DLBCL; B-cells; GCB DLBCL; ABC (non-GCB) DLBCL; CDT1; DNMT1; PCNA; MCM2; MCM7; EZH2

Sažetak
Diffuse large B-cell lymphoma (DLBCL) is a neoplasm of B-cells with characteristic diffuse pattern of distribution. It accounts for approximately 30% of non-Hodgkin lymphomas in elderly. DLBCL can be stratified, based on gene gene expression profiling, into GCB and ABC (non-GCB) subtypes. ABC DLBCL indicates more agressive clinical course than GCB group. In most DLBCL cases, large number of genetic rearrangements can be detected. The most frequent is BCL6 translocation, followed by translocations of BCL2 and c-MYC. The aim of this study was to analyse changes in amount of proteins involved in replication process and chromatin reassembly mechanisms in DLBCL subtypes. These changes might be responsible for aberrations in replication accuracy which further leads to accumulation of additional mutations in tumor cells causing tumor progression. DLBCL cases were classified into GCB and non-GCB subtyes using immunohistochemical algorithm by Hans et al. RNA from all samples was extracted and converted to cDNA by reverse transcription. q- RT PCR analysis of DNMT1, PCNA, MCM2, MCM7, GMNN, CDT1, EZH2 and p300 expression showed increased expression of CDT1 and DNMT1 as well as decreased expression of PCNA, MCM2, MCM7 and EZH2 in GCB when compared with non-GCB subtype. Results suggest contribution of replication and chromatin reassembly machinery components to DLBCL pathogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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