Naslov
DONOR SPECIFIC ANTIBODY MONITORING AND CLINICAL OUTCOME IN KIDNEY TRANSPLANTATION

Autori
Katalinić, Nataša ; Živčić-Ćosić, Stela ; Fućak, Marina ; Starčević, Alma ; Crnić, Tajana ; Kurtović, Helena ; Mujić, Aida ; Ćurković, Milena ; Duhović, Marijana ; Balen, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
BANTAO 12th DIATRANSPLANT 2015

Mjesto i datum
Opatija, Hrvatska, 15.10.2015. - 18.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
kidney transplantation; donor specific antibodies; Luminex; allograft survival

Sažetak
PURPOSE: Allograft failure and rejection are associated with the presence of donor specific antibodies (DSA) in the recipient's serum. Detection of low levels of HLA-specific antibodies is enabled by the development of more sensitive methods such as solid phase immunoassays (ELISA, Luminex) and flow cytometry. We analyzed the results of DSA detection by Luminex technique with the clinical outcome after kidney transplantation. MATERIALS AND METHODS: We performed retrospective DSA testing (Immucor) in pre-transplant (day 0) and post-transplant sera samples (1 month) of 35 adult renal allograft recipients in University Hospital Center Rijeka, transplanted between November 2009 and October 2012. In all recipients, the pre-transplant cytotoxicity crossmatch was negative. RESULTS: DSA testing revealed median fluorescence intensity (MFI) >2000 in two sera samples (2.9%), MFI 1000-2000 in two samples (2.9%), MFI 700-1000 in four samples (5.7%), while all other samples were negative. All DSA reactivity was related to the HLA Class II antibodies. Rejection episodes were observed in two patients with positive DSA results in pre-transplant sera (MFI 996 and 6092, respectively). Two patients with DSA positivity in pre-transplant sera (MFI >2000) and negative test result one month after transplantation, rejected the allograft within the first year after transplantation. CONCLUSIONS: DSA reactivity with MFI levels above 2000 in pre-transplant sera can be considered as a predictive factor for allograft rejection. To provide more reliable data, the postransplant monitoring should be performed during a longer follow-up period.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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