Naslov
Excision of Visceral Adipose Tissue from Live Mice (VATectomy)

Autori
Šestan, Marko ; Wensveen, Felix M. ; Polić, Bojan

Izvornik
Bio-protocol (2331-8325) 5 (2015), 23; 1668-1668

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, ostalo, ostalo

Ključne riječi
Murine visceral adipose tissue; Tissue excision

Sažetak
The visceral adipose tissue (VAT) has been shown to play an important role in various biological functions. It is a storage depot for nutrients and it is an important endocrine organ producing hormones that control systemic metabolism (McGown et al., 2014). Importantly, following diet-induced obesity, VAT accumulates a large number of activated immune cells, which produce cytokines that drive chronic systemic inflammation and promote insulin resistance (Johnson et al., 2012 ; Wensveen et al., 2015). VAT therefore plays a key role in metabolic, endocrinological and immunological research. To show the importance of this organ in various research models, one may surgically remove the organ in a procedure called VATectomy. This protocol describes the technical procedures required for an efficient VATectomy of the perigonadal fat pads in mice.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA