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Pregled bibliografske jedinice broj: 810791

A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR

White, Helen; Deprez, L; Corbisier, P; Hall, V; Lin, F; Mazoua, S; Trapmann, S; Aggerholm, A; Andrikovics, H; Akiki, S et al.
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR // Leukemia, 29 (2015), 2; 369-376 doi:10.1038/leu.2014.217 (podatak o recenziji nije dostupan, članak, ostalo)

CROSBI ID: 810791 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR

Autori
White, Helen ; Deprez, L ; Corbisier, P ; Hall, V ; Lin, F ; Mazoua, S ; Trapmann, S ; Aggerholm, A ; Andrikovics, H ; Akiki, S ; Barbany, G ; Boeckx, N ; Bench, A ; Catherwood, M ; Cayuela, JM ; Chudleigh, S ; Clench, T ; Colomer, D ; Daraio, F ; Dulucq, S ; Farrugia, J ; Fletcher, L ; Foroni, Letizia ; Ganderton, R ; Gerrard G, Gineikienė E, Hayette S, El Housni, H ; Izzo, B ; Jansson, M ; Johnels P, Jurcek T, Kairisto V, Kizilors A, Kim DW, Lange T, Lion T, Polakova KM, Martinelli G, McCarron S, Merle, PA ; Milner B, Mitterbauer- Hohendanner, Gerlinde ; Nagar, M ; Nickless, G ; Nomdedéu, J ; Nymoen, DA ; Leibundgut, EO ; Ozbek, U ; Pajič, Tadej ; Pfeifer, H ; Preudhomme, C ; Raudsepp, K ; Romeo, G ; Sacha, T ; Talmaci, R ; Touloumenidou, T ; Van der Velden, VH ; Waits, P ; Wang, L ; Wilkinson, E ; Wilson, G ; Wren, D ; Zadro, Renata ; Ziermann, J ; Zoi, K ; Müller ; Martin ; Hochhaus, Andreas ; Schimmel, Heinz, Cross, Nicholas ; Emons, H.

Izvornik
Leukemia (0887-6924) 29 (2015), 2; 369-376

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
certified plasmid reference material ; bcr-abl1

Sažetak
Serial quantification of BCR–ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukaemia, but there is a substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR– ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08 ± 0.13 × 106, 1.08 ± 0.11 × 105, 1.03 ± 0.10 × 104, 1.02 ± 0.09 × 103, 1.04 ± 0.10 × 102 and 10.0 ± 1.5 copies/μl. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR–ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise a number of measured transcripts of e14a2 BCR–ABL1 and three control genes (ABL1, BCR and GUSB). The set of six plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (https://ec.europa. eu/jrc/en/reference- materials/catalogue/ ; CRM code ERM-AD623a-f).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Profili:

Avatar Url Danko Müller (autor)

Avatar Url Renata Zadro (autor)

Poveznice na cjeloviti tekst rada:

doi www.nature.com

Citiraj ovu publikaciju:

White, Helen; Deprez, L; Corbisier, P; Hall, V; Lin, F; Mazoua, S; Trapmann, S; Aggerholm, A; Andrikovics, H; Akiki, S et al.
A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR // Leukemia, 29 (2015), 2; 369-376 doi:10.1038/leu.2014.217 (podatak o recenziji nije dostupan, članak, ostalo)
White, H., Deprez, L., Corbisier, P., Hall, V., Lin, F., Mazoua, S., Trapmann, S., Aggerholm, A., Andrikovics, H. & Akiki, S. (2015) A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR. Leukemia, 29 (2), 369-376 doi:10.1038/leu.2014.217.
@article{article, author = {White, Helen and Deprez, L and Corbisier, P and Hall, V and Lin, F and Mazoua, S and Trapmann, S and Aggerholm, A and Andrikovics, H and Akiki, S and Barbany, G and Boeckx, N and Bench, A and Catherwood, M and Cayuela, JM and Chudleigh, S and Clench, T and Colomer, D and Daraio, F and Dulucq, S and Farrugia, J and Fletcher, L and Foroni, Letizia and Ganderton, R and Izzo, B and Jansson, M and Nagar, M and Nickless, G and Nomded\'{e}u, J and Nymoen, DA and Leibundgut, EO and Ozbek, U and Paji\v{c}, Tadej and Pfeifer, H and Preudhomme, C and Raudsepp, K and Romeo, G and Sacha, T and Talmaci, R and Touloumenidou, T and Van der Velden, VH and Waits, P and Wang, L and Wilkinson, E and Wilson, G and Wren, D and Zadro, Renata and Ziermann, J and Zoi, K and Hochhaus, Andreas and Emons, H.}, year = {2015}, pages = {369-376}, DOI = {10.1038/leu.2014.217}, keywords = {certified plasmid reference material, bcr-abl1}, journal = {Leukemia}, doi = {10.1038/leu.2014.217}, volume = {29}, number = {2}, issn = {0887-6924}, title = {A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR}, keyword = {certified plasmid reference material, bcr-abl1} }
@article{article, author = {White, Helen and Deprez, L and Corbisier, P and Hall, V and Lin, F and Mazoua, S and Trapmann, S and Aggerholm, A and Andrikovics, H and Akiki, S and Barbany, G and Boeckx, N and Bench, A and Catherwood, M and Cayuela, JM and Chudleigh, S and Clench, T and Colomer, D and Daraio, F and Dulucq, S and Farrugia, J and Fletcher, L and Foroni, Letizia and Ganderton, R and Izzo, B and Jansson, M and Nagar, M and Nickless, G and Nomded\'{e}u, J and Nymoen, DA and Leibundgut, EO and Ozbek, U and Paji\v{c}, Tadej and Pfeifer, H and Preudhomme, C and Raudsepp, K and Romeo, G and Sacha, T and Talmaci, R and Touloumenidou, T and Van der Velden, VH and Waits, P and Wang, L and Wilkinson, E and Wilson, G and Wren, D and Zadro, Renata and Ziermann, J and Zoi, K and Hochhaus, Andreas and Emons, H.}, year = {2015}, pages = {369-376}, DOI = {10.1038/leu.2014.217}, keywords = {certified plasmid reference material, bcr-abl1}, journal = {Leukemia}, doi = {10.1038/leu.2014.217}, volume = {29}, number = {2}, issn = {0887-6924}, title = {A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR}, keyword = {certified plasmid reference material, bcr-abl1} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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