Naslov
The grain yield and quality of winter wheat cultivars under FHB pressure, content of Fusarium mycotoxins -­ understanding the resistance by antioxidant pathways

Autori
Španić, Valentina ; Abičić, Ivan ; Marček, Tihana ; Vijevac Vuletić, Marija ; Horvat, Daniela ; Tucak Marijana ; Šarkanj, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of Abstracts 5th International Symposium on Fusarium Head Blight 2nd International Workshop on Wheat Blast / Del Ponte Emerson, M ; Carlton Bergstrom, G ; Pavan, W ; Lazzaretti, A ; Fernandes Cuha, J.M. - Passo Fundo : Ed Universidade de Passo Fundo, 2016, 20-20

Skup
5th International Symposium on Fusarium Head Blight 2nd International Workshop on Wheat Blast

Mjesto i datum
Florianópolis, Brazil, 06.04.2016. - 09.04.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
wheat ; grain yield ; grain quality ; mycotoxins ; antioxidant compounds

Sažetak
Fusarium head blight (FHB) is a significant wheat disease severely limiting crop productivity, which is caused by fungi that can synthesize toxic components. Human or animal exposure to mycotoxins can harm their health and in some cases cause death, because of carcinogenic or cytotoxic activity or impairment of the immune system. Because this is also widespread disease in Croatia in three year project we want to analyse the impact of Fusarium spp. infection on yield components and protein quality parameters, showing the degradation of wheat proteins by reversed-­‐ ‑phase high-­‐‑performance liquid chromatography (HPLC) and the effect on processing quality parameters. The LC-­‐‑MS/MS method in this study will be applied for the determination of mycotoxins in four different treatments (seed and malt in control and in inoculated treatment). Also we will check some antioxidant compounds (guaiacol peroxidase, ascorbate peroxidase, superoxide dismutase, catalase, free proline and malondialdehyde content) which can influence the fungal redox status playing an important role during trichothecene biosynthesis. In preliminary results the genotypes Srpanjka and Olimpija showed the highest resistance level for resistance component I. Fusarium-­‐‑colonized kernels were ranking from 3 to 45% in inoculated treatment, which was in correlation with AUDPC for general resistance. The best yields in the inoculated treatment were retained by genotypes Vulkan and Srpanjka which didn't had any relative grain yield loss. Differences for grain yield between the two treatments (control and inoculation) have exceeded 40%. Lower yields were accompanied with lower test weights and higher 1, 000 kernel weight. The technological quality parameters, such as gluten index and falling number, were less affected by the Fusarium than grain yield, but anyway decrease of protein content, wet gluten content and sedimentation value was obvious. Gliadins were influenced by FHB-­‐‑ in one wheat cultivar by decreasing more then 25%. On the contrary, we observed a slight increase in total gliadin content and gliadin subfractions in severely Fusarium-­‐‑infected kernels. In contrast, the content of total glutenins was significantly reduced, except in one cultivar. Consequently, gliadin-­‐‑to-­‐ ‑glutenin ratio was increased in the infected samples, while an increase in a albumins and globulins (up to 50%) was observed. In control group deoxynivalenol (DON) was present in 1 of 6 wheat seed samples (51.64 ng/ml) and 2 of 6 wheat malt samples. In inoculated treatment all samples were positive with DON (51.2 to 3, 246.53 ng/ml). The most susceptible cultivar had the highest 3-­‐‑ADON accumulation in kernels. Zearalenone was present in 5 of 6 malt samples in inoculated treatment (17.80-­‐‑232.57 ng/ml). HT-­‐‑2 toxin occurred in two samples in control (31.7 ng/ml and 33.42 ng/ml), and in one sample in inoculated treatment (30.94 ng/ml). This work has been supported in part by Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA