Naslov
The dupA genes in Helicobacter pyloriand their relationship with gastroduodenal diseases in North-west Croatia

Autori
Varda-Brkić, Dijana ; Presečki-Stanko, Aleksandra ; Žele-Starčević, Lidija ; Tripković, Vesna ; Mareković, Ivana ; Frančula-Zanović, Sonja ; Bedenić, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
26th European Congress of Clinical Microbiology and Infectious Diseases

Mjesto i datum
Amsterdam, Nizozemska, 09.04.2016. - 12.04.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dupA; Helicobacter pylori; gastroduodenal diseases

Sažetak
ABSTRACT Background: Duodenal ulcer promoting gene A (dupA) is a virulence factor of Helicobacter pylori that is associated with severe antral inflammation and high acid secretion, as well as, protection against atrophy, intestinal metaplasia, and gastric cancer, and thus, it can be considered as a disease-specific virulence marker. The prevalence of dupA in H. pylori in patients with gastritis worldwide differed significantly among nationality and ethnicity. Most studies did not found significant association between the presence of dupA in H. pylori strains and gastroduodenal disorders. Although each population may have been too small to reveal a true effect. This might be the reason why the importance of dupA in H. pylori on clinical outcomes had conflicting results between different studies. Aim: The aim of our study was to determine the presence of dupA gene in H. pylori isolates and evaluate the association between dupA and endoscopic and pathohistological findings in patients from Northwestern Croatia. Materials/Methods: In total, 103 H. pylori isolates were analyzed. The presence of dupA (jhp0917 and jhp0918 genes) was detected by polymerase chain reaction (PCR). According to the endoscopic findings patients were classified in three groups: non ulcer dyspepsia NUD (n=68), erosio/ulcus ventriculi EUV (n=22), erosio/ulcus duodeni EUD (n=13). Gastric pathohistological changes were recorded according to the updated Sydney system. Results: Of 103 patients included in the study 25 were men and 78 woman in the age range of 28 to 81 years (median 55.8 years). DupA gene was detected in 35 (34.0%) H. pylori isolates. Thirty five (34.0%) and 57 (55.3%) of the 103 tested isolates, respectively, were positive and negative for both genes. The remaining 11 (10.7%) of the 103 strains were jhp0917- positive/jhp0918-negative. The frequency of dupA genes in H. pylori isolates was significantly different (p=0.016) between the subgroups according to endoscopic diagnosis, highest in NUD (28/68), comparable in EUV (7/22), and none in EUD (0/13). It was significantly lower in patients with the high intensity score for antral gastritis (p=0.041), but not significant different between patients with different intensity scores for corporal gastritis (p=0.338). No significant difference in frequency of dupA genes was found between subgroups according to gastritis type predominance (antral-dominant, corporal- dominant, diffuse gastritis, p=0.691). Conclusion: We haven’t found any positive dupA genes in H. pylori isolates in EUD. Significant reverse association was found between the frequency of dupA in H. pylori isolates and intensity score for antral gastritis. Also no difference was found for frequency of dupA in H. pylori isolates regarding the predominance of gastritis (antral vs. corporal). The limitations of our study come from a small number of EUD cases and the absence of gastric cancer GC cases.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA