Naslov
Chronic iron overload induces gender-specific changes in iron homeostasis, lipid peroxidation and clinical course of experimental autoimmune encephalomyelitis

Autori
Ćurko-Cofek, Božena ; Grubić-Kezele, Tanja ; Marinić, Jelena ; Tota, Marin ; Starčević Čizmarević, Nada ; Milin, Čedomila ; Ristić, Smiljana ; Radošević-Stašić, Biserka ; Barac-Latas, Vesna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Interactive-program, The 10th International Congress on Autoimmunity, Leipzig / - , 2016, EP09 E-Posters

Skup
The 10th International Congress on Autoimmunity

Mjesto i datum
Leipzig, Njemačka, 06.04.2016. - 10.04.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
iron overload; EAE; gender

Sažetak
Background/Objectives: Iron is an essential element for cell functions, such as cell proliferation, respiration, folate metabolism and DNA synthesis, having a crucial role in metabolic pathways involved in electron transfer and ATP production. The redox potential of Fe2+/Fe3+ favors its use in a number of protein complexes, but ferrous iron via the Fenton reaction may induce the production of extremely reactive hydroxyl radicals that damage DNA, proteins and lipids. Design/Method: Using experimental autoimmune encephalomyelitis, as an animal model of multiple sclerosis (MS) in this study we analyzed the effects of iron overload on kinetics of disease, iron status and lipid peroxidation. For this purpose female and male DA rats were treated by iron sucrose (75 mg/kg bw/day) or with saline solution during two weeks before the sensitization with bovine brain homogenate in complete Freund's adjuvant. Serum ferritin and tissue contents of iron and malondialdehyde (MDA) were determined in the central nervous system and in the liver on day 13 after immunization. Results: Iron overload in female rats accelerated the onset and first peak of disease, increased the content of ferritin and induced an accumulation of Fe (liver and brain) and MDA (liver). In contrast, in male rats it accelerated the second relapse, augmented iron content (liver) and MDA (spinal cord and brain), and increased the mortality rate. Conclusions: The data point to sexual dimorphism in mechanisms that regulate peripheral and brain iron homeostasis and imply that men and women during MS might be differentially vulnerable to exogenous iron overload.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA