Naslov
Neuronal differentiation in the developing human spinal ganglia

Autori
Vukojević, Katarina ; Filipović, Natalija ; Tica Sedlar, Ivana ; Restović, Ivana ; Bočina, Ivana ; Pintarić, Irena ; Saraga-Babić, Mirna

Izvornik
Anatomical record-advances in integrative anatomy and evolutionary biology (1932-8486) 299 (2016), 8; 1060-1072

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human embryo ; spinal ganglia ; IB4 ; NF200 ; CGRP ; VR1

Sažetak
The spatiotemporal developmental pattern of the neural crest cells differentiation towards the first appearance of the neuronal subtypes was investigated in developing human spinal ganglia between the 5th-10th developmental week using immunohistochemistry and immunofluorescence methods. First NF200 (neurofilament-200, likely- myelinated mechanoreceptors) and isolectin-B4 positive neurons (likely- unmyelinated nociceptors) appeared already in the 5/6th developmental week and their number subsequently increased during progression of development. Proportion of NF200 positive cells was higher in the ventral parts of the spinal ganglia than in the dorsal parts, particularly during the 5/6th and 9/10th developmental weeks (Mann-Whitney, p=0.040 and p=0.003). NF200 and IB4 co-localized during the whole investigated period. Calcitonin gene-related peptide (CGRP, nociceptive responses), vanilloid-receptor-1 (VR1, polymodal nociceptors) and calretinin (calcium signalling) cell immunoreactivity first appeared in the 6th and 8th week, respectively, especially in the dorsal parts of the spinal ganglia. VR1 and CGRP co-localized with NF00 during the whole investigated period. Our results indicate the high potential of early differentiated neuronal cells, which slightly decreased with progression of spinal ganglia differentiation. On the contrary, the number of neuronal subtypes displayed increasing differentiation at later developmental stage. The great diversity of phenotypic expression found in the spinal ganglia neurons is the result of a wide variety of influences, occurring at different stages of development in a large potential repertory of these neurons. Understanding the pathway of neural differentiation in the human spinal ganglia could be important for the studies dealing with process of regeneration of damaged spinal nerves or during repair of pathological changes within affected ganglia.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA