Naslov
Solution-state structure of a G-quadruplex formed within the promoters of Plasmodium falciparum var genes

Autori
Juribašić Kulcsar, Marina ; Plavec, Janez

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
FIBER-SLONMR symposium / - , 2015

Skup
The first joint FIBER-SLONMR symposium: Frontiers on nucleic acid structure and function

Mjesto i datum
Celje, Slovenija, 05.10.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
G-quadruplex ; nucleic acid ; long loop ; NMR spectroscopy ; malaria

Sažetak
G-quadruplexes formed by guanine-rich (G-rich) nucleic acid sequences influence many key biological functions thus being in the spotlight of chemical and biological investigations, pharmaceutics and nanotechnology. Structures consist of Hoogsteen-bonded guanines forming G-quartets connected by loops which prolongation reduces the stability of the structure. We report NMR high-resolution solution structure of an intramolecular DNA G-quadruplex with unprecedented ten-nucleotides long loop which breaks the traditional loop length dogma of maximum seven nucleotides expected for maintaining a stable structure. 34-nt long sequence, d[CAG3T2A2G3TATA2CT3AG4T2AG3T2], found within promoters of Plasmodium falciparum var genes, that play a key role in malaria pathogenesis and immune evasion, folds under physiological conditions (150 mM K+ and pH 7.0) into a structurally unique [3+1]-hybrid structure extensively stabilized by non-standard hydrogen bonding and base stacking.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA