Naslov
Solution-state structure of a G-quadruplex formed within promoters of Plasmodium falciparum B var genes

Autori
Juribašić Kulcsar, Marina ; Plavec, Janez

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
7th NMR research workshop 2015 / - , 2015

Skup
7th NMR research workshop, NMR structural studies: From organic compounds to biological macromolecules

Mjesto i datum
Idrija, Slovenija, 03.12.2015. - 04.12.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
G-quadruplex ; nucleic acid ; long loop ; NMR spectroscopy ; malaria

Sažetak
G-quadruplexes formed by guanine-rich (G-rich) nucleic acid sequences influence many key biological functions thus being in the spotlight of chemical and biological investigations, pharmaceutics and nanotechnology. Structures consist of Hoogsteen-bonded guanines forming G-quartets connected by loops which prolongation reduces the stability of the structure. We report NMR high-resolution solution structure of an intramolecular DNA G-quadruplex with unprecedented ten-nucleotides long loop which breaks the traditional loop length dogma of maximum seven nucleotides expected for maintaining a stable structure. 34-nt long sequence, d[CAG3T2A2G3TATA2CT3AG4T2AG3T2], found within promoters of Plasmodium falciparum var genes, that play a key role in malaria pathogenesis and immune evasion, folds under physiological conditions (150 mM K+ and pH 7.0) into a structurally unique [3+1]-hybrid structure extensively stabilized by non-standard hydrogen bonding and base stacking.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA