Naslov
PREPARATION OF AN ESTERASE

Autori
Kietzmann, Martin ; Schwab, Helmut ; Pichler, Harald ; Ivancic, Mirela, May, Oliver ; Luiten, Rudolf Gijsbertus Marie

Broj patenta
WO 2009004093

Godina
2011

Datum patenta
21.07. 2011.

Nositelj prava
DSM IP Assets B.V. ; NL-6411 TE Heerlen (NL), ,

Sažetak
The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA