Naslov
The active site mutants of yeast seryl-tRNA synthetase

Autori
Lenhard, B., Landeka, I., Filipić, S., Weygand- Đurašević, I.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual Meeting of Croatian Biochemists with International Participation / Željko Kućan - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 1995, 50-50

ISBN
953-6256-16-9

Skup
Annual Meeting of Croatian Biochemists with International Participation

Mjesto i datum
Supetar, Hrvatska, 22.09.1995. - 25.09.1995

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
seryl-tRNA synthetase; yeast

Sažetak
Aminoacyl-tRNA synthetases are responsible for the specific aminoacylation of transfer RNAs. According to their structural features, the members of this enzyme family can be partitioned into two classes. The active site domain of all so far crystallized class II aminoacyl-tRNA synthetases exhibit an original fold, constituted by several antiparallel beta- sheets, connected by the loops of variable lenghts. In order to study structure/function relationship in yeast seryl-tRNA synthetase (SerRS), which has not been crystallized yet, a number of mutants have been generated by extensive in vitro mutagenesis of SES1 gene and subjected to functional in vivo and in vitro analysis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA