Naslov
Effect of Propolis on Diet-Indused Hyperlipidemia in Mice

Autori
Landeka, I., Đikić, D., Teparić, R., Oršolić, N., Rogić, D.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
8th International Conference Functional Foods for Chronic Diseases, University of Nevada

Mjesto i datum
Las Vegas (NV), Sjedinjene Američke Države, 15.03.2011. - 17.03.2011

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
propolis; lipoproteins; cholesterol; triglycerides; HDL-cholesterol; LDL-cholesterol

Sažetak
Background: One of the main environmental factors causing obesity in human is the intake of a high-fat diet. Lipoproteins consist of lipids and proteins called apoproteins. Obesity resulting from an accumulation of visceral adiposity is a primary cause of ˝metabolic syndrome˝ that is the combination of multiple cardiovascular risk factors, including visceral obesity, dyslipidemia, glucose intolerance, and hypertension. Propolis possesses many biological activities, such as antitumor, antioxidant, antimicrobial, anti-inflammatory, and immunomodulatory, among others. A diet- induced obesity animal model has been developed to investigate human obesity. Recent studies have reported that propolis prevented and mitigated diabetes and hypertension, but it remains unclear whether propolis impinge on the hyperlipidemia and liver functions. Methods: In this study we examined the effect of propolis on liver functions and hyperlipidemia induced in a high-fat diet induced obesity mouse model. The propolis extract, at dose of 50 mg/kg body weight/day, was given, by gauge, to mice for 30 days. At the end of the treatment, Results: The mice on an oil diet had higher (P £0.05) weight gain than the control and all other experimental groups (FIG. 1). Propolis applied together with the oil-high fat diet reduced weight gain of experimental animals (P £0.05). A marked reduction (P£0.05) was observed in the amount of serum lipids in animals on combined high fat diet and propolis. Similar lipid reduction (P£0.05) was noted in liver total triglycerides in group oil+propolis group. LDL cholesterol levels in serum significantly ascend in oil receiving group. However the LDL cholesterol level in groups supplemented with propolis was significantly lower (P£0.05) than in the group that was receiving oil in their diet. The basis of LDL/HDL cholesterol ratio the calculated atherogenic index was significantly elevated (P £0.05) in the group that was receiving oil. Atherogenic index in the group that was receiving oil and propolis diminished significantly (P£0.05) in comparison to the groups treated with oil alone. Propolis alone did not change the atherogenic index significantly. Major serum metabolites were elevated due to the oil diet. Namely, significantly higher levels (P£0.05) of glucose, creatinine, uric acid and protein level were noted in mice receiving oil compared to controls. Similarly as in other measured parameters glucose, creatinine, uric acid and protein level in mice receiving oil and propolis was reduced than in mice treated only with oil. Enzyme activities in serum of mice given oil with propolis also followed the pattern of metabolites, indicating that the overall metabolic turnover increased due to the higher energetic level. A significant decrease (P£0.05) was observed in serum enzymes, such as aminotransferase (EC 2.6.1.2), phosphatase (EC 3.1.3.1), dehydrogenase (EC 1.1.1.27) activities of mice given oil and propolis. Conclusions: Propolis caused lower weight gain in animals on a high fat diet. In this mouse model of induced obesity propolis improved lipid profile of animals on a high fat diet. Propolis has a potential to prevent hyperlipidemia. All measured effects have to be evaluated by further research in a same high fat diet model using each individual polyphenolic components of propolis in order to conclude whether the reduced lypidemia is caused by propolis as a complex mixture of flavonoides or individual components.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA