Pregled bibliografske jedinice broj: 803004
The application of the tape stripping technique for the determination of silver penetration into the skin of AD patients and healthy controls
The application of the tape stripping technique for the determination of silver penetration into the skin of AD patients and healthy controls // 4th International Summer Academy of Practical Dermatology
München, Njemačka, 2015. (predavanje, međunarodna recenzija, sažetak, znanstveni)
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Naslov
The application of the tape stripping technique for the determination of silver penetration into the skin of AD patients and healthy controls
Autori
Jakasa I, Pluut OA, Bianco C, Visser MJ, Krystek P, Larese-Filon F, Rustemeyer T, Kezic S
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
4th International Summer Academy of Practical Dermatology
Mjesto i datum
München, Njemačka, 28.07.2015. - 31.07.2015
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Tape stripping; percutaneous penetration; dermal exposure; atopic dermatitis
Sažetak
Due to its antimicrobial properties silver has been increasingly used for medical purposes e.g in wound care to combat skin infections in patients with atopic dermatitis (AD) or contact dermatitis. However, little is known on the amount of silver that can penetrate across the skin which might lead to systemic adverse effects. In particular, in vivo studies on dermal absorption of silver through diseased skin are lacking. The main objective of the present study was to explore the possibility to determine percutaneous penetration of silver by using stratum corneum tape stripping technique. Next, to determine dermal absorption through healthy and atopic skin and compare it with current reference values. 20 healthy subjects and 20 patients with AD wore a sleeve containing 3.6% (w/w) silver on their lower arms for 8 h during 5 consecutive days. Dermal flux (mg/cm2/hr) was deduced from the silver concentration-depth profiles in the SC collected by adhesive tapes. The silver concentration in the SC tapes has been determined by ICP-MS technique. Dermal exposure of silver resulted in low but measurable amounts of silver in the SC even after the first 8 h of exposure revealing that silver is able to penetrate into and across the SC. Dermal flux of silver was 0.20 and 0.23 ng/cm2/h in the AD patients and healthy volunteers, respectively. Dermal absorption of silver under “real life scenario” was lower than the current reference dose. The developed tape stripping technique can also be used for the determination of other metals e.g. contact allergens like nickel.
Izvorni jezik
Engleski
