Naslov
Comparison of high power ultrasound and cold plasma assisted extraction of glycosides from Stevia (Stevia rebaudiana)

Autori
Režek Jambrak, Anet ; Kujundžić, Dijana ; Vukušić, Tomislava ; Herceg, Zoran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 1st World Congress on Electroporation and Pulsed Electric Fields / - , 2015

Skup
The 1st World Congress on Electroporation and Pulsed Electric Fields

Mjesto i datum
Portorož, Slovenija, 06.09.2015. - 10.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
high power ultrasound; cold plasma; water extraction; glycosides; CCD

Sažetak
Non-thermal processing techniques like high power ultrasound and cold plasma are used in many processes to improve, shorten and to optimize food processing. These techniques are also used for extraction of bioactive compounds from food products and by-products (waste), in order to increase yield of extractive compounds and to obtain the most stable extraction procedure. The extraction procedure of rebaudioside A and stevioside from Stevia was set as experimental design procedure. Extraction were performed in water (GRAS-generally recognised as safe), which makes this GREEN process extraction. Using central composite design (CCD), 3 factors of ultrasound extraction parameters were varied (amplitude, temperature and treatment time) and plasma extraction parameters (gas flow, treatment time and volume of sample). Parallel to non-thermal procedures, Soxhlet extraction was performed (3h and 6h). Output values were concentration of rebaudioside A and stevioside (mg/mL) and the yield (mg/g). Glycosides were detected and quantified using HPLC procedure. The results showed that high power ultrasound treatment gave better output results in concentration of glycosides and yield (5.1-6.2 mg/ml ; yield 102-122 mg/g) compared to cold plasma treatment (4.8-5.4 mg/ml ; yield 96-109 mg/g). Also, ultrasound and plasma treatment had higher yield and concentration of glycosides compared to Soxhlet extraction for 6h (3.5 mg/ml ; yield 70 mg/g), and for 3h (3.6 mg/ml ; yield 72 mg/g). Novel non-thermal techniques are causing microstreaming and cavitation in the liquid, thereby causing softening of plant tissue and opening the cells, which causes easier and faster extraction.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA