Naslov
Acid base balance as a predictor of metabolic or oxidative stress in periparturient dairy cows

Autori
Đidara, Mislav ; Šperanda, Marcela ; Pavić, Mirela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the First DairyCare Conference 2014 / Knight, C. H. - Kopenhagen : DairyCare COST Action FA1308, 2014, 40-40

ISBN
978-0-9930176-0-5

Skup
Health and Welfare of Dairy Animals

Mjesto i datum
Danska, 22.08.2014. - 23.08.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Acid base balance ; Oxidative stress ; Dairy cows

Sažetak
Metabolic and oxidative stress in transition period is marked by elevated (NEFA, βHB, GPx, SOD, AST, ALT) or decreased (Ca, GSH) level of many metabolites and enzymes in the blood. Question remains if acid-base parameters can be used as the predictors of metabolic or oxidative stress during transition period. Experiment was conducted on 30 dairy Holstein cows, from 3±1 weeks before parturition until the 6th week of lactation. Cows were allocated into three groups of 10: the control (C) group was fed with standard diet for dry period and lactation. Cows in the first experimental group (LS) were fed with diet in which most of the soybean was replaced with the linseed. Cows from the second experimental group (LS+Se) were fed with the LS group diet plus 0.3 ppm of organic selenium replacement (B-Traxim®, Pancosma, Switzerland). On days -10, 1, 21 and 42 relative to parturition blood was collected and analysed. Parameters of metabolic diseases (NEFA, βHB, AST, ALT, Ca) did not differ significantly (p>0.05) between groups. NEFA and βHB concentrations were significantly (p<0.05) higher on day 1 indicating metabolic pressure, because of NEB during transition period. Oxidative stress during transition period was marked by the significantly (p<0.05) higher SOD and GPx activity on day 1. The MDA level was higher (p<0.05) in the both experimental groups. However, the MDA decreased (p<0.05) in all groups during the period after parturition. Parameters of acid-base balance (pH, pCO2, pO2, HCO3-, BE and anion gap) did not differ between groups or during time (p>0.05). Statistical analyse of those parameters on day 1 (when metabolic and oxidative stress has been most pronounced) shows that there were correlations (p<0.05) between pH : urea (-0.83) ; pO2 : GSH (0.85) and AnGap:urea (0.78) in the control group ; pH : MDA (-0.96) ; pCO2 : βHB (-0.82) ; pO2 : βHB (0.93) and HCO3- : βHB (-0.82) in the LS group and pO2 : MDA (-0.97) and pO2 : glucose (0.99) in the LS+Se group. Despite different diet conditions, some of the acid- base parameters like pH, pO2, AnGap, HCO3, could be used as predictors of metabolic stress in dairy cows.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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