Naslov
Characterization of photosynthetic picoeukaryotes from the Southern Adriatic Pit
(Charactrization of photosynthetic picoeukaryotes from the Southern Adriatic Pit)

Autori
Mejdandžić, Maja ; Orlić, Sandi ; Bosak, Sunčica ; Bošnjak, Ivana ; Cetinić, Ivona ; Peharec Štefanić, Petra ; Sesar, Tihana ; Gašparović, Blaženka ; Ljubešić, Zrinka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of papers presented at the EMBO | EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes / Nürnberger, Tim - Heidelberg : Mera Druck GmbH, Sandhausen, Germany, 2016, 170-170

Skup
EMBO | EMBL Symposium: A New Age of Discovery for Aquatic Microeukaryotes

Mjesto i datum
Heidelberg, Njemačka, 26.01.2016. - 29.01.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
photosynthetic picoeukaryotes ; Picoclorum ; Nannochloris ; Southern Adriatic Pit

Sažetak
Photosynthetic picoeukaryotes with cell size less than 3 μm are widely distributed in the world oceans and play critical role in oceanic primary production. In recent years, the composition and abundance of marine picoeukaryotes have been investigated using several approaches: morphological (LM, TEM) and chemical studies, flow-cytometry studies, chemotaxonomic and molecular studies. Adriatic Sea, the northern most part of Mediterranean is semi-enclosed oligotrophic basin where picophytoplankton is of an extreme importance for the functionality of the ecosystem. Until recently, our knowledge about phytoplankton community in Adriatic was limited to micro- and nanophytoplankton, while pico-fraction except of bacteria was neglected. During BIOTA cruise in February/March 2015, along two transects in Southern Adriatic pit, several clones of picoeukaryotes were isolated, cultivated and analysed using imaging, chemical and molecular methods. Photosynthetic picoeukaryotes were isolated from stations where flow cytometry analysis revealed their high abundances at greater depths (>280 m) well below photosynthesis active irradiance (PAR) values. Photosynthetic picoeukaryote plastid 16S rRNA gene was amplified with one set of primers - PLA491F/OXY1313R. Sequence and BLAST analysis of 4 monoclonal strains revealed 99-100% similarity with genera Nannochloris and Picochlorum, and some uncultured eukaryote clones. Lipid composition demonstrated clear domination of glycolipid sulfoquinovosyldiacylglycerol, on average 34% among all lipid classes. Combination of morphological, chemical, ecological and phylogenetic data allowed us to characterise Nannochloris-like microalgae from Southern Adriatic Pit.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

POVEZANOST RADA