Naslov
OPTIMIZATION OF METHODS FOR MOLECULAR SEX TYPING IN HERONS (ARDEIDAE)

Autori
Mikuška, Alma ; Begović, Lidija ; Mihić, Ivan ; Kljajić, Kristina ; Grgac, Robert

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka (Hrvatski biološki kongres s međunarodnim sudjelovanjem) / Klobučar, Göran ; Kopjar, Nevenka ; GligoraUdovič, Marija ; Lukša, Žaklin ; Jelić, Dušan - Zagreb : Hrvatsko biološko društvo, 2015, 151-151

Skup
12. HRVATSKI BIOLOŠKI KONGRES s međunarodnim sudjelovanjem

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 18.09.2015. - 23.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
genetic markers; sex‐typing; herons

Sažetak
Genetic markers are useful tool for tracking changes in population studies. This work, is focused on optimization of method for DNA isolation and molecular sex determination in herons (Ardeidae). Genomic DNA was isolated from feathers and eggshells by using two different protocols. For sex‐typing of herons we used primers 2550F/2781R for chromo‐helicase–DNA binding gene 1 (CHD1). Results showed that the highest DNA yield was obtained from the blood feathers and eggshells while mature feathers had the lowest yield. DNA yield was lower when commercial kit was used for isolation compared to the isolation with TNE buffer followed by addition of sodium acetate for keratin precipitation. Critical step is concentration of dithiothreitol (DTT) and proteinase K in lysis solution as well as the amount of blood present in the feathers.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA