Naslov
Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores

Autori
Kajtez, Janko ; Solomatina, Anastasia ; Novak, Maja ; Polak, Bruno ; Vukušić, Kruno ; Rüdiger, Jonas ; Cojoc, Gheorge ; Milas, Ana ; Šumanovac Šestak, Ivana ; Risteski, Patrik ; Tavano, Federica ; H. Klemm, Anna ; Roscioli, Emanuele ; Welburn, Julie ; Cimini, Daniela ; Glunčić, Matko ; Pavin, Nenad ; Tolić, Iva Marija

Izvornik
Nature Communications (2041-1723) 7 (2016); 10298, 11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Kinetochores ; Microtubules ; Mitotic Spindle ; Metaphase

Sažetak
During metaphase, forces on kinetochores are exerted by k fibres, bundles of microtubules that end at the kinetochore. Interestingly, non-kinetochore microtubules have been observed between sister kinetochores, but their function is unknown. Here we show by laser- cutting of a k-fibre in HeLa and PtK1 cells that a bundle of non- kinetochore microtubules, which we term ‘bridging fibre’, bridges sister k-fibres and balances the interkinetochore tension. We found PRC1 and EB3 in the bridging fibre, suggesting that it consists of antiparallel dynamic microtubules. By using a theoretical model that includes a bridging fibre, we show that the forces at the pole and at the kinetochore depend on the bridging fibre thickness. Moreover, our theory and experiments show larger relaxation of the interkinetochore distance for cuts closer to kinetochores. We conclude that the bridging fibre, by linking sister k-fibres, withstands the tension between sister kinetochores and enables the spindle to obtain a curved shape.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

POVEZANOST RADA