Naslov
Characterization of a S-adenosyl-L-methionine (SAM)-accumulating strain of Scheffersomyces stipitis

Autori
Križanović, Stela ; Butorac, Ana ; Mrvčić, Jasna ; Krpan, Maja ; Cindrić, Mario ; Bačun-Družina, Višnja ; Stanzer, Damir

Izvornik
International microbiology (1139-6709) 18 (2015), 3; 117-125

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Scheffersomyces stipitis ; S-adenosyl-L-methionine (SAM) ; SAM accumulating yeast ; C-24 sterol methyltrans-ferase (Erg6p)

Sažetak
S-adenosyl-L-methionine (SAM) is an important molecule in the cellular metabolism of mammals. In this study, we examined several of the physiological characteristics of a SAM-accumulating strain of the yeast Scheffersomyces stipitis (M12), including SAM production, ergosterol content, and ethanol tolerance. S. stipitis M12 accumulated up to 52.48 mg SAM/g dry cell weight. Proteome analyses showed that the disruption of C-24 methylation in ergosterol biosynthesis, a step mediated by C-24 sterol methyltransferase (Erg6p), and results in greater SAM accumulation by S. stipitis M12 compared to the wild-type strain. A comparative proteome-wide analysis identified 25 proteins that were differentially expressed by S. stipitis M12. These proteins are involved in ribosome biogenesis, translation, the stress response, ubiquitin-dependent catabolic processes, the cell cycle, ethanol tolerance, posttranslational modification, peroxisomal membrane stability, epigenetic regulation, the actin cytoskeleton and cell morphology, iron and copper homeostasis, cell signalling, and energy metabolism.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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