Naslov
The European antibody network's practical guide to finding and validating suitable antibodies for research.

Autori
Roncador G ; Engel P ; Maestre L, Anderson AP ; Cordell JL ; Cragg MS ; Šerbec VČ ; Jones M, Juranić Lisnić, Vanda ; Kremer, L ; Li D ; Koch-Nolte F ; Pascual N ; Rodríguez-Barbosa JI ; Torensma R ; Turley H ; Pulford K ; Banham AH.

Izvornik
MAbs (1942-0862) E-pub ahead of print (2015), E-pub ahead of print;

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
BLAST searches ; CiteAb ; EuroMAbNet ; flow cytometry ; gene silencing ; HLDA workshops ; immunohistochemistry ; monoclonal antibodies ; validation studies ; western blotting

Sažetak
Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody- based data fit for publication.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA