Naslov
Influence of solid-liquid extraction conditions on the proteins extraction from barley grains

Autori
Bucić-Kojić, Ana ; Strelec, Ivica ; Planinić, Mirela ; Koceva Komlenić, Daliborka ; Tomas, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th International Congress Flour-Bread '15, 10th Croatian Congress of Cereal Technologists, Book of abstracts / Koceva Komlenić, Daliborka ; Jukić, Marko - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2015

Skup
8th International Congress Flour-Bread '15, 10th Croatian Congress of Cereal Technologists

Mjesto i datum
Opatija, Hrvatska, 29.10.2015. - 30.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
extraction; barley grains; protein recovery; optimization

Sažetak
Influence of extraction temperature (30-90 °C), concentration of ethanol/water solution (30-90%, v/v) and time (10-70 min) on extractability of proteins from barley grains was examined. Barley grain samples were collected from three different locations throughout Croatia: Nova Gradiška (sample BNG), Čakovec (sample BC) and Vinkovci (sample - BV). Box-Bhenken design of Response surface methodology (RSM) was used for identification of the best extraction conditions and extraction yield optimization. Total protein content (PC) in extracts was determined by the Bradford method, PCBR and Lowry method, PCLO. RSM analysis showed that ethanol concentration and extraction temperature had significant effect, while effect of time and interaction of factors on the observed extraction process were not significant. The optimal extraction conditions were achieved at 90 °C, 60%, v/v ethanol/water and 60 min with predicted maximum protein recovery, PCBR of 15.28 mgBSA/gdb, 16.74 mgBSA/gdb and 20.48 mgBSA/gdb for BNG, BC and BV, respectively. At the same extraction conditions, predicted maximum protein recovery, PCLO were 51.33 mgBSA/gdb, 57.64 mgBSA/gdb, and 56.90 mgBSA/gdb for BNG, BC and BV, respectively.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA